Könyvfejezetekhttp://repo.lib.semmelweis.hu//handle/123456789/882024-03-29T12:17:42Z2024-03-29T12:17:42ZThe impact of the COVID-19 pandemic on important relationships in HungaryHerke, BoglárkaAlbert, FruzsinaBrys, ZoltánGerdán, Mercédeszhttp://repo.lib.semmelweis.hu//handle/123456789/96722024-02-19T11:19:42Z2023-01-01T00:00:00ZThe impact of the COVID-19 pandemic on important relationships in Hungary
Herke, Boglárka; Albert, Fruzsina; Brys, Zoltán; Gerdán, Mercédesz
2023-01-01T00:00:00ZChronic obstructive pulmonary disease and smokersBikov, AndrásLázár, Zsófiahttp://repo.lib.semmelweis.hu//handle/123456789/85042022-09-01T05:57:37Z2020-01-01T00:00:00ZChronic obstructive pulmonary disease and smokers
Bikov, András; Lázár, Zsófia
2020-01-01T00:00:00ZMMP Activity Detection in ZymogramsBencsik, PéterBartekova MGörbe, AnikóKiss, KrisztinaPálóczi, JánosSzűcs, GergőFerdinandy, Péterhttp://repo.lib.semmelweis.hu//handle/123456789/65122022-07-01T08:56:21Z2017-01-01T00:00:00ZMMP Activity Detection in Zymograms
Bencsik, Péter; Bartekova M; Görbe, Anikó; Kiss, Krisztina; Pálóczi, János; Szűcs, Gergő; Ferdinandy, Péter
Matrix metalloproteinases (MMP) belong to a distinguished class of zinc-dependent endopeptidases. Zymography is a semi-quantitative tool for determining the activity of different MMP isoenzymes in a variety of biological samples. In substrate gel zymography, protein samples of different origin (tissue, cell lysates, plasma/serum, perfusates, other liquids) are separated in sodium dodecyl sulfate (SDS) polyacrylamide gels containing copolymerized substrate (gelatin, casein, elastin, etc.), and after incubation-enabling substrate cleavage by MMPs, MMP activities are detected after the gel staining as transparent bands against a dark-blue background. In situ zymography is a histological modification of substrate zymography in frozen sections, allowing detection of the localization of the MMP activities within the tissue. Here, we describe detailed experimental protocols of all abovementioned techniques and provide examples for several sample measurements.
2017-01-01T00:00:00ZThe Signaling Nature of Cellular Metabolism: The Hypoxia SignalingFabian, Zsolthttp://repo.lib.semmelweis.hu//handle/123456789/70142022-02-10T08:48:07Z2019-01-01T00:00:00ZThe Signaling Nature of Cellular Metabolism: The Hypoxia Signaling
Fabian, Zsolt
2019-01-01T00:00:00Z