http://repo.lib.semmelweis.hu//handle/123456789/26 Wed, 29 Jul 2026 03:35:29 GMT 2026-07-29T03:35:29Z http://repo.lib.semmelweis.hu//handle/123456789/10445 Development of a Novel Human Serum Albumin-Based Tool for Effective Drug Discovery: The Investigation of Protein Quality and Immobilization Kenéz, Balázs; Koplányi, Gábor; Decsi, Balázs; Molnár, Zsófia; Horváth, Péter; Katona, Gábor; Balogh, György T.; Balogh-Weiser, Diána The binding ability of human serum albumin (HSA) on active pharmaceutical ingredients (APIs) is one of the most important parameters in the early stages of drug discovery. In this study, an immobilized HSA-based tool was developed for the rapid and easy in vitro screening of API binding. The work explored the serious incompleteness in the identification of HSA used for in vitro screening published in the last five years. To mitigate this problem, a comprehensive analysis and immobilization studies were performed on the most used HSA types. Serious differences in the colloidal stability of HSAs and their API binding ability on a selected set of APIs were observed. HSAs were immobilized on magnetic nanoparticles with glutardialdehyde (GDA) or cyclohexyl-diglycidyl ether (CDGE) linkers, which have never been used for HSA immobilization before. The HSA-MNP-CDGE complexes achieved a higher immobilization yield and preserved API binding ability; however, the esterase-like enzymatic activity of HSA reduced significantly. Wed, 01 Jan 2025 00:00:00 GMT http://repo.lib.semmelweis.hu//handle/123456789/10445 2025-01-01T00:00:00Z http://repo.lib.semmelweis.hu//handle/123456789/10416 Tudomány és könyvtár egy életben – Marton János (1943–2018) Vasas, Lívia Thu, 01 Jan 2026 00:00:00 GMT http://repo.lib.semmelweis.hu//handle/123456789/10416 2026-01-01T00:00:00Z http://repo.lib.semmelweis.hu//handle/123456789/10418 Elhízás és túlsúly előfordulása 1-es típusú diabetes mellitus miatt gondozott gyermekeknél az elmúlt 10 év adatai alapján Muzslay, Eszter; Pusztai, Rozália Ildikó; Forstreuter, Vilmos Zoltán; Herczeg, Vivien; Szabó, Attila József; Tóth-Heyn, Péter; Luczay, Andrea Thu, 01 Jan 2026 00:00:00 GMT http://repo.lib.semmelweis.hu//handle/123456789/10418 2026-01-01T00:00:00Z http://repo.lib.semmelweis.hu//handle/123456789/10415 A hajléktalanság, mint közegészségügyi probléma a COVID-19 járvány idején Magyarországon Horti-Maricza, Zsuzsanna; Simek, Ágnes Wed, 01 Jan 2025 00:00:00 GMT http://repo.lib.semmelweis.hu//handle/123456789/10415 2025-01-01T00:00:00Z