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dc.contributor.author Bencsik, Péter
dc.contributor.author Bartekova M
dc.contributor.author Görbe, Anikó
dc.contributor.author Kiss, Krisztina
dc.contributor.author Pálóczi, János
dc.contributor.author Szűcs, Gergő
dc.contributor.author Ferdinandy, Péter
dc.date.accessioned 2022-07-01T08:54:22Z
dc.date.available 2022-07-01T08:54:22Z
dc.date.issued 2017
dc.identifier 85020803452
dc.identifier.citation pagination=53-70; journalVolume=1626; journalTitle=METHODS IN MOLECULAR BIOLOGY;
dc.identifier.uri http://repo.lib.semmelweis.hu//handle/123456789/6512
dc.identifier.uri doi:10.1007/978-1-4939-7111-4_6
dc.description.abstract Matrix metalloproteinases (MMP) belong to a distinguished class of zinc-dependent endopeptidases. Zymography is a semi-quantitative tool for determining the activity of different MMP isoenzymes in a variety of biological samples. In substrate gel zymography, protein samples of different origin (tissue, cell lysates, plasma/serum, perfusates, other liquids) are separated in sodium dodecyl sulfate (SDS) polyacrylamide gels containing copolymerized substrate (gelatin, casein, elastin, etc.), and after incubation-enabling substrate cleavage by MMPs, MMP activities are detected after the gel staining as transparent bands against a dark-blue background. In situ zymography is a histological modification of substrate zymography in frozen sections, allowing detection of the localization of the MMP activities within the tissue. Here, we describe detailed experimental protocols of all abovementioned techniques and provide examples for several sample measurements.
dc.relation.ispartof urn:issn:1064-3745
dc.title MMP Activity Detection in Zymograms
dc.type Journal Article
dc.date.updated 2018-09-25T14:25:37Z
dc.language.rfc3066 en
dc.identifier.mtmt 3240023
dc.identifier.pubmed 28608200


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