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dc.contributor.author Grozdics Enikő
dc.contributor.author Berta László
dc.contributor.author Bajnok Anna
dc.contributor.author Veres Gábor
dc.contributor.author Ilisz István
dc.contributor.author Klivényi Péter
dc.contributor.author Rigó János
dc.contributor.author Vécsei László
dc.contributor.author Tulassay Tivadar
dc.contributor.author Toldi Gergely
dc.date.accessioned 2015-02-05T13:04:32Z
dc.date.available 2015-02-05T13:04:32Z
dc.date.issued 2014
dc.identifier.citation pagination=306-; journalVolume=14; journalTitle=BMC PREGNANCY AND CHILDBIRTH;
dc.identifier.uri http://repo.lib.semmelweis.hu//handle/123456789/1110
dc.identifier.uri doi:10.1186/1471-2393-14-306
dc.description.abstract BACKGROUND: B7 costimulatory molecules are expressed on antigen presenting cells (APCs) and are important regulators of T cell activation. We investigated the role of the B7 family of costimulatory molecules in the development of the systemic maternal immune tolerance during healthy pregnancy (HP). We also aimed to investigate the intracellular expression of indoleamine-2,3-dioxygenase (IDO) and plasma levels of tryptophane (TRP), kynurenine (KYN) and kynurenic acid (KYNA), important molecules with immunoregulatory properties, in order to describe their potential contribution to the pregnancy-specific maternal immune tolerance. METHODS: We determined the frequency of activated (CD11b+) monocytes expressing B7-1, B7-2, B7-H1, and B7-H2, and that of T cells and CD4+ T helper cells expressing CD28, CTLA-4, PD-1, and ICOS in peripheral blood samples of healthy pregnant (HP) and non-pregnant (NP) women using flow cytometry. We also examined the intracellular expression of IDO applying flow cytometry and plasma levels of TRP, KYN and KYNA using high-performance liquid chromatography. RESULTS: A significant increase in the prevalence of CD28+ T cells was observed in HP compared to NP women. At the same time a decrease was shown in the expression of CTLA-4 on these cells. The frequency of CD80+ monocytes was lower in HP women. The prevalence of IDO-expressing T cells and monocytes was higher in HP compared to NP women. Plasma KYN, KYNA and TRP levels were lower, while at the same time, the KYN/TRP ratio was higher in HP than in NP women. CONCLUSIONS: Costimulation via CD28 may not contribute to the immunosuppressive environment, at least in the third trimester of pregnancy. The development of the pregnancy-specific immune tolerance in the mechanism of B7 costimulation may be more related to the altered expression of B7 proteins on APCs rather than that of their receptors on T cells. The elevated intracellular IDO expression in monocytes and T cells, as well as higher plasma enzymatic IDO activity are likely to contribute to the systemic immunosuppressive environment in the third trimester characteristic for healthy gestation.
dc.relation.ispartof urn:issn:1471-2393
dc.title B7 costimulation and intracellular indoleamine-2,3-dioxygenase (IDO) expression in peripheral blood of healthy pregnant and non-pregnant women.
dc.type Journal Article
dc.date.updated 2015-01-19T09:24:39Z
dc.language.rfc3066 en
dc.identifier.mtmt 2732682
dc.identifier.wos 000341396800004
dc.identifier.pubmed 25189405
dc.contributor.department SE/AOK/K/I. Sz. Gyermekgyógyászati Klinika
dc.contributor.department SE/AOK/K/I. Sz. Szülészeti és Nőgyógyászati Klinika
dc.contributor.department SE/AOK/K/ISZGYK/MTA-SE Gyermekgyógyászati és Nephrológiai Kutatócsoport
dc.contributor.institution Semmelweis Egyetem


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