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dc.contributor.author Gonzalez, Sara
dc.contributor.author Manzanares, Jorge
dc.contributor.author Berrendero, Fernando
dc.contributor.author Wenger, Tibor
dc.contributor.author Corchero, Javier
dc.contributor.author Tiziana, Bisogno
dc.contributor.author Julian, Romero
dc.contributor.author José A, Fuentes
dc.contributor.author Vincenzo Di, Marzo
dc.contributor.author José A, Ramos
dc.contributor.author Javier, Fernandez-Ruiz
dc.date.accessioned 2016-09-15T08:42:41Z
dc.date.available 2016-09-15T08:42:41Z
dc.date.issued 1999
dc.identifier.citation pagination=137-145; journalVolume=70; journalIssueNumber=2; journalTitle=NEUROENDOCRINOLOGY;
dc.identifier.uri http://repo.lib.semmelweis.hu//handle/123456789/1426
dc.description.abstract Most data on effects of natural and synthetic cannabinoids on anterior pituitary hormone secretion point out to a primary impact on the hypothalamus. There is also some evidence, however, of possible direct actions of these compounds on the anterior pituitary, although the presence of cannabinoid receptors in the pituitary has not been documented as yet. In the present study, we evaluated the presence of cannabinoid CB(1) receptor-mRNA transcripts in the pituitary gland by in situ hybridization. We observed CB(1) receptor-mRNA transcripts in the anterior pituitary and to a lesser extent in the intermediate lobe whereas they were absent in the neural lobe. We then examined whether CB(1) receptor-mRNA levels in both pituitary lobes responded to chronic activation by a specific agonist, as did receptors located in adjacent hypothalamic nuclei and in other brain regions. Daily administration of CP-55,940 for 18 days produced a small, but statistically significant paradoxical increase in CB(1) receptor-mRNA levels in the anterior pituitary, with no changes in the intermediate lobe, in contrast to reduced CB(1) receptor-mRNA levels observed in the ventromedial hypothalamic nucleus (VMN), and to decreased CB(1) receptor binding in the VMN and the arcuate nucleus. The time-course of up-regulation of CB(1) receptor-mRNA transcripts in the anterior lobe was biphasic; daily administration of Delta(9)-tetrahydrocannabinol produced an early and marked decrease in CB(1) receptor-mRNA levels after 1 and 3 days, followed by normalization after 7 days and by a small increase after 14 days. We also checked whether endogenous cannabinoid ligands are present in the anterior pituitary and the hypothalamus. Although anandamide itself was detected only in trace amounts, concentrations of its precursor N-arachidonoyl-phosphatidyl-ethanolamine and of 2-arachidonoyl-glycerol were found in both tissues, suggesting that endocannabinoids may be synthetized in the anterior pituitary. In summary, CB(1) receptors and corresponding ligands seem to be expressed in cells of the anterior and intermediate lobes of the pituitary, but the response of CB(1) receptor-mRNA transcripts in the anterior lobe to chronic agonist activation is different than the desensitization observed in hypothalamic nuclei.
dc.relation.ispartof urn:issn:0028-3835
dc.title Identification of endocannabinoids and cannabinoid CB(1) receptor mRNA in the pituitary gland
dc.type Journal Article
dc.date.updated 2015-02-18T12:35:40Z
dc.language.rfc3066 en
dc.identifier.mtmt 1506963
dc.identifier.pubmed 10461028
dc.contributor.department SE/AOK/I/Humánmorfológiai és Fejlődésbiológiai Intézet
dc.contributor.institution Semmelweis Egyetem


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