dc.contributor.author |
Dudás József |
|
dc.contributor.author |
Fullár Alexandra |
|
dc.contributor.author |
Mario Bitsche |
|
dc.contributor.author |
Volker Schartinger |
|
dc.contributor.author |
Kovalszky Ilona |
|
dc.contributor.author |
Georg Mathias Sprinzl |
|
dc.contributor.author |
Herbert Riechelmann |
|
dc.date.accessioned |
2016-01-06T13:29:22Z |
|
dc.date.available |
2016-01-06T13:29:22Z |
|
dc.date.issued |
2011 |
|
dc.identifier |
79960844181 |
|
dc.identifier.citation |
pagination=2222-2229;
journalVolume=317;
journalIssueNumber=15;
journalTitle=EXPERIMENTAL CELL RESEARCH; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/1672 |
|
dc.identifier.uri |
doi:10.1016/j.yexcr.2011.05.023 |
|
dc.description.abstract |
Recently we described a co-culture model of periodontal ligament (PDL) fibroblasts and SCC-25 lingual squamous carcinoma cells, which resulted in conversion of normal fibroblasts into carcinoma-associated fibroblasts (CAFs), and in epithelial-mesenchymal transition (EMT) of SCC-25 cells. We have found a constitutive high interleukin-1beta (IL1-beta) expression in SCC-25 cells in normal and in co-cultured conditions. In our hypothesis a constitutive IL1-beta expression in SCC-25 regulates gene expression in fibroblasts during co-culture. Co-cultures were performed between PDL fibroblasts and SCC-25 cells with and without dexamethasone (DEX) treatment; IL1-beta processing was investigated in SCC-25 cells, tumor cells and PDL fibroblasts were treated with IL1-beta. IL1-beta signaling was investigated by western blot and immunocytochemistry. IL1-beta-regulated genes were analyzed by real-time qPCR. SCC-25 cells produced 16kD active IL1-beta, its receptor was upregulated in PDL fibroblasts during co-culture, which induced phosphorylation of interleukin-1 receptor-associated kinase-1 (IRAK-1), and nuclear translocalization of NFkappaBalpha. Several genes, including interferon regulatory factor 1 (IRF1) interleukin-6 (IL-6) and prostaglandin-endoperoxide synthase 2 (COX-2) were induced in CAFs during co-culture. The most enhanced induction was found for IL-6 and COX-2. Treatment of PDL fibroblasts with IL1-beta reproduced a time- and dose-dependent upregulation of IL1-receptor, IL-6 and COX-2. A further proof was achieved by DEX inhibition for IL1-beta-stimulated IL-6 and COX-2 gene expression. Constitutive expression of IL1-beta in the tumor cells leads to IL1-beta-stimulated gene expression changes in tumor-associated fibroblasts, which are involved in tumor progression. |
|
dc.relation.ispartof |
urn:issn:0014-4827 |
|
dc.title |
Tumor-produced, active interleukin-1beta regulates gene expression in carcinoma-associated fibroblasts. |
|
dc.type |
Journal Article |
|
dc.date.updated |
2015-04-08T13:54:51Z |
|
dc.language.rfc3066 |
en |
|
dc.identifier.mtmt |
2877198 |
|
dc.identifier.wos |
000293682400013 |
|
dc.identifier.pubmed |
21664353 |
|
dc.contributor.department |
SE/AOK/I/I. Sz. Patológiai és Kísérleti Rákkutató Intézet |
|
dc.contributor.institution |
Semmelweis Egyetem |
|