dc.contributor.author |
Yu-Fan Hsieh |
|
dc.contributor.author |
Guang-Yaw Liu |
|
dc.contributor.author |
Yi-Ju Lee |
|
dc.contributor.author |
Jiann-Jou Yang |
|
dc.contributor.author |
Sándor Katalin |
|
dc.contributor.author |
Sarang Zsolt |
|
dc.contributor.author |
Angela Bononi |
|
dc.contributor.author |
Paolo Pinton |
|
dc.contributor.author |
Tretter László |
|
dc.contributor.author |
Szondy Zsuzsanna |
|
dc.contributor.author |
Gregory J Tsay |
|
dc.date.accessioned |
2014-07-17T07:32:59Z |
|
dc.date.available |
2014-07-17T07:32:59Z |
|
dc.date.issued |
2013 |
|
dc.identifier.citation |
pagination=e81516;journalVolume=8;journalIssueNumber=12;journalTitle=PLOS ONE; |
hu |
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/267 |
|
dc.identifier.uri |
doi:10.1371/journal.pone.0081516 |
|
dc.description.abstract |
BACKGROUND:
Transglutaminase 2 (TG2) is a protein cross-linking enzyme known to be associated with the in vivo apoptosis program of T cells. However, its role in the T cell apoptosis program was not investigated yet.
RESULTS:
Here we report that timed overexpression of both the wild type (wt) and the cross-linking mutant of TG2 induced apoptosis in Jurkat T cells, the wt being more effective. Part of TG2 colocalised with mitochondria. WtTG2-induced apoptosis was characterized by enhanced mitochondrial Ca(2+) uptake. Ca(2+)-activated wtTG2 cross-linked RAP1, GTP-GDP dissociation stimulator 1, an unusual guanine exchange factor acting on various small GTPases, to induce a yet uncharacterized signaling pathway that was able to promote the Ca(2+) release from the endoplasmic reticulum via both Ins3P and ryanodine sensitive receptors leading to a consequently enhanced mitochondrial Ca(2+)uptake.
CONCLUSIONS:
Our data indicate that TG2 might act as a Ca(2+) sensor to amplify endoplasmic reticulum-derived Ca(2+) signals to enhance mitochondria Ca(2+) uptake. Since enhanced mitochondrial Ca(2+) levels were previously shown to sensitize mitochondria for various apoptotic signals, our data demonstrate a novel mechanism through which TG2 can contribute to the induction of apoptosis in certain cell types. Since, as compared to knock out cells, physiological levels of TG2 affected Ca(2+) signals in mouse embryonic fibroblasts similar to Jurkat cells, our data might indicate a more general role of TG2 in the regulation of mitochondrial Ca(2+) homeostasis. |
hu |
dc.relation.ispartof |
urn:issn:1932-6203 |
|
dc.title |
Transglutaminase 2 Contributes to Apoptosis Induction in Jurkat T Cells by Modulating Ca(2+) Homeostasis via Cross-Linking RAP1GDS1 |
hu |
dc.type |
Journal Article |
hu |
dc.date.updated |
2014-07-17T06:42:00Z |
|
dc.language.rfc3066 |
en |
hu |
dc.identifier.mtmt |
2505661 |
|
dc.identifier.pubmed |
24349085 |
|
dc.contributor.department |
SE/ÁOK/I/Orvosi Biokémiai Intézet |
|
dc.contributor.department |
SE/ÁOK/I/OBI/MTA-SE Neurobiokémiai Kutatócsoport |
|
dc.contributor.institution |
Semmelweis Egyetem |
|
dc.mtmt.swordnote |
FELTÖLTŐ: Csajbók Edit - ecsajbok@lib.sote.hu |
|