Show simple item record Kunz P Fellenberg J Moskovszky, Linda Sápi, Zoltán Krenács, Tibor Machado I Poeschl J Lehner B Szendrői, Miklós Ruef P Bohlmann M Bosch AL Ewerbeck V Kinscherf R Fritzsching B 2016-02-09T11:26:38Z 2016-02-09T11:26:38Z 2015
dc.identifier 84906439521
dc.identifier.citation pagination=489-496; journalVolume=22; journalIssueNumber=2; journalTitle=ANNALS OF SURGICAL ONCOLOGY;
dc.identifier.uri doi:10.1245/s10434-014-4001-2
dc.description.abstract BACKGROUND: Osteosarcoma is considered a highly vascularized bone tumor with early metastatic dissemination through intratumoral blood vessels mostly into the lung. Novel targets for therapy such as tumor vascularization are highly warranted since little progress has been achieved in the last 30 years. However, proof of relevance for vascularization as a major prognostic parameter has been hampered by tumor heterogeneity, difficulty in detecting microvessels by immunohistochemistry, and small study cohorts. Most recently, we demonstrated that highly standardized whole-slide imaging could overcome these limitations (Kunz et al., PloS One 9(3):e90727, 2014). In this study, we applied this method to a multicenter cohort of 131 osteosarcoma patients to test osteosarcoma vascularization as a prognostic determinant. METHODS: Computer-assisted whole-slide analysis, together with enzymatic epitope retrieval, was used for CD31-based microvessel quantification in 131 pretreatment formalin-fixed and paraffin-embedded biopsies from three bone tumor centers. Kaplan-Meier-estimated survival and chemoresponse were determined and multivariate analysis was performed. Conventional hot-spot-based microvessel density (MVD) determination was compared with whole-slide imaging. RESULTS: We detected high estimated overall (p </= 0.008) and relapse-free (p </= 0.004) survival in 25 % of osteosarcoma patients with low osteosarcoma vascularization in contrast to other patient groups. Furthermore, all patients with low osteosarcoma vascularization showed a good response to neoadjuvant chemotherapy. Comparison of conventional MVD determination with whole-slide imaging suggests false high quantification or even exclusion of samples with low osteosarcoma vascularization due to difficult CD31 detection in previous studies. CONCLUSION: Low intratumoral vascularization at the time of diagnosis is a strong predictor for prolonged survival and good response to neoadjuvant chemotherapy in osteosarcoma.
dc.relation.ispartof urn:issn:1068-9265
dc.title Improved Survival in Osteosarcoma Patients with Atypical Low Vascularization.
dc.type Journal Article 2015-11-26T08:49:54Z
dc.language.rfc3066 en
dc.identifier.mtmt 2747925
dc.identifier.wos WOS:000347817900023
dc.identifier.pubmed 25155396
dc.contributor.department SE/AOK/I/I. Sz. Patológiai és Kísérleti Rákkutató Intézet
dc.contributor.department SE/AOK/K/Ortopédiai Klinika
dc.contributor.institution Semmelweis Egyetem

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