Abstract:
The production and the composition of the compounds (mono-,
sesquiterpenes and acetylenic thiophenes) obtained by the steam
distillation of Tagetes patula L. have been investigated. The volatile
oil was produced by steam distillation. GC was carried out on three
types of stationary phase using flame ionization and mass selective
detection. Percentage data were calculated by the area normalization
method with very good repeatability (RSD below 5%). Oils from
flowerheads were rich in beta-caryophyllene (53.5%) and the leaves
contained terpinolene in high concentration (21.1%). The main volatile
component of the hairy roots and intact roots was
5(3-buten-1-ynyl)-2,2'-bithienyl (BBT) yielding 28.5% and 44.0% in the
oils. Three new minor constituents were identified as alpha-guriunene,
beta-caryophyllene and (E)-beta-farnesene. A flash chromatographic
method was developed for the isolation of thiophenes from a solvent
extract of intact roots. The collected fractions were screened by TLC
and analyzed by GC-MS. Three thiophene fractions were obtained
containing BBT, 5-(4-acetoxy-1-butynyl)-2,2'-bithienyl (BBTOAc) and
5-(4-hydroxy-1-butynyl)-2,2'-bithienyl (BBTOH).