Kivonat:
Lobelia inflato L. contains pharmacologically important secondary
metabolites. The main alkaloid is lobeline which has a stimulatory
effect on the respiratory centre. Recently high quantity of
polyacetylenes was determined in the plant and hairy root cultures.
With the aim of increasing of the secondary metabolite production hairy
root cultures were produced (by direct infection of the sterile plants
with Agrobacterium rhizogenes strain R1601). A new HPLC method was
developed for the determination of lobetyolin and lobetyolinin, the
main polyacetylene compounds of L. inflata hairy roots. The analysis
was performed on Hypersil MOS column (250 x 4 mm I.D.). The mobile
phase was 15:85 (v/v) mixture of acetonitrile and water. Peaks were
identified by addition of standards and by DAD. The external
standardized method allowed the determination of lobetyolin and
lobetyolinin with good sensitivity and reliability. This method was
used to measure the polyacetylene contents of hairy roots grew
intensively in bioreactor. High contents of lobetyolin (3.6%) and
lobetyolinin (0.8-1.6%) were detected in the hairy roots because of
their great biosynthetic capacity.