Show simple item record Hoda MA, Munzker J, Ghanim B, Schelch K, Klikovits T, Laszlo, Viktoria Sahin E Bedeir A Lackner A Döme, Balázs Setinek U Filipits M Eisenbauer M Kenessey, István Török Szilvia Garay, Tamás Hegedűs, Balázs Catania A Taghavi S Klepetko W Berger W Grusch M 2016-11-18T11:22:39Z 2016-11-18T11:22:39Z 2012
dc.identifier 84870818616
dc.identifier.citation pagination=1978-1986; journalVolume=107; journalIssueNumber=12; journalTitle=BRITISH JOURNAL OF CANCER;
dc.identifier.uri doi:10.1038/bjc.2012.519
dc.description.abstract Background:Activins control the growth of several tumour types including thoracic malignancies. In the present study, we investigated their expression and function in malignant pleural mesothelioma (MPM).Methods:The expression of activins and activin receptors was analysed by quantitative PCR in a panel of MPM cell lines. Activin A expression was further analysed by immunohistochemistry in MPM tissue specimens (N=53). Subsequently, MPM cells were treated with activin A, activin receptor inhibitors or activin-targeting siRNA and the impact on cell viability, proliferation, migration and signalling was assessed.Results:Concomitant expression of activin subunits and receptors was found in all cell lines, and activin A was overexpressed in most cell lines compared with non-malignant mesothelial cells. Similarly, immunohistochemistry demonstrated intense staining of tumour cells for activin A in a subset of patients. Treatment with activin A induced SMAD2 phosphorylation and stimulated clonogenic growth of mesothelioma cells. In contrast, treatment with kinase inhibitors of activin receptors (SB-431542, A-8301) inhibited MPM cell viability, clonogenicity and migration. Silencing of activin A expression by siRNA oligonucleotides further confirmed these results and led to reduced cyclin D1/3 expression.Conclusion:Our study suggests that activin A contributes to the malignant phenotype of MPM cells via regulation of cyclin D and may represent a valuable candidate for therapeutic interference.
dc.relation.ispartof urn:issn:0007-0920
dc.title Suppression of activin A signals inhibits growth of malignant pleural mesothelioma cells
dc.type Journal Article 2016-06-09T09:36:35Z
dc.language.rfc3066 en
dc.identifier.mtmt 2172184
dc.identifier.wos 000312495300010
dc.identifier.pubmed 23169291
dc.contributor.department SE/AOK/I/II. Sz. Patológiai Intézet
dc.contributor.institution Semmelweis Egyetem
dc.mtmt.swordnote PMC PMC3516694

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