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dc.contributor.author Turu Gábor
dc.contributor.author Várnai Péter
dc.contributor.author Gyombolai Pál
dc.contributor.author Szidonya László
dc.contributor.author Offertaler L
dc.contributor.author Bagdy György
dc.contributor.author Hunyady László
dc.date.accessioned 2018-03-22T17:57:18Z
dc.date.available 2018-03-22T17:57:18Z
dc.date.issued 2009
dc.identifier 67650564947
dc.identifier.citation pagination=16914-16921; journalVolume=284; journalIssueNumber=25; journalTitle=JOURNAL OF BIOLOGICAL CHEMISTRY;
dc.identifier.uri http://repo.lib.semmelweis.hu//handle/123456789/5187
dc.identifier.uri doi:10.1074/jbc.M109.003681
dc.description.abstract Intracellular signaling systems of G protein-coupled receptors are well established, but their role in paracrine regulation of adjacent cells is generally considered as a tissue-specific mechanism. We have shown previously that AT(1) receptor (AT(1)R) stimulation leads to diacylglycerol lipase-mediated transactivation of co-expressed CB(1)Rs in Chinese hamster ovary cells. In the present study we detected a paracrine effect of the endocannabinoid release from Chinese hamster ovary, COS7, and HEK293 cells during the stimulation of AT(1) angiotensin receptors by determining CB(1) cannabinoid receptor activity with bioluminescence resonance energy transfer-based sensors of G protein activation expressed in separate cells. The angiotensin II-induced, paracrine activation of CB(1) receptors was visualized by detecting translocation of green fluorescent protein-tagged beta-arrestin2. Mass spectrometry analyses have demonstrated angiotensin II-induced stimulation of 2-arachidonoylglycerol production, whereas no increase of anandamide levels was observed. Stimulation of G(q/11)-coupled M(1), M(3), M(5) muscarinic, V(1) vasopressin, alpha(1a) adrenergic, B(2) bradykinin receptors, but not G(i/o)-coupled M(2) and M(4) muscarinic receptors, also led to paracrine transactivation of CB(1) receptors. These data suggest that, in addition to their retrograde neurotransmitter role, endocannabinoids have much broader paracrine mediator functions during activation of G(q/11)-coupled receptors.
dc.relation.ispartof urn:issn:0021-9258
dc.title Paracrine transactivation of the CB1 cannabinoid receptor by AT1 angiotensin and other Gq/11 protein-coupled receptors.
dc.type Journal Article
dc.date.updated 2018-03-19T10:48:42Z
dc.language.rfc3066 en
dc.identifier.mtmt 1235701
dc.identifier.wos 000266962400028
dc.identifier.pubmed 19357084
dc.contributor.department SE/AOK/I/Élettani Intézet
dc.contributor.department MTA-SE Neurobiokémiai és Molekuláris Élettani Kutatócsoport (2006-ig: MTA-SE Neurobiokémiai Kutatócsoport) [2006.12.31]
dc.contributor.department SE/GYTK/Gyógyszerhatástani Intézet
dc.contributor.department SE/GYTK/GYHATAS/MTA-SE Neuropszichofarmakológiai és Neurokémiai Kutatócsoport
dc.contributor.institution Semmelweis Egyetem
dc.contributor.institution MTA Támogatott Kutatócsoportok


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