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dc.contributor.author Varga, Zoltán
dc.contributor.author Zvara, Ágnes
dc.contributor.author Faragó, Nóra
dc.contributor.author Kocsis-Fodor, Gabriella
dc.contributor.author Pipicz, Márton
dc.contributor.author Gáspár, Renáta
dc.contributor.author Bencsik, Péter
dc.contributor.author Görbe, Anikó
dc.contributor.author Csonka, Csaba
dc.contributor.author Puskás, László
dc.contributor.author Thum T
dc.contributor.author Csont, Tamás Bálint
dc.contributor.author Ferdinandy, Péter
dc.date.accessioned 2018-08-29T15:44:13Z
dc.date.available 2018-08-29T15:44:13Z
dc.date.issued 2014
dc.identifier 84904335129
dc.identifier.citation pagination=H216-H227; journalVolume=307; journalIssueNumber=2; journalTitle=AMERICAN JOURNAL OF PHYSIOLOGY: HEART AND CIRCULATORY PHYSIOLOGY;
dc.identifier.uri http://repo.lib.semmelweis.hu//handle/123456789/6169
dc.identifier.uri doi:10.1152/ajpheart.00812.2013
dc.description.abstract We aimed to characterize early changes in microRNA expression in acute cardioprotection by ischemic pre- and postconditioning in rat hearts. Hearts isolated from male Wistar rats were subjected to i) time-matched non-ischemic perfusion, ii) ischemia/reperfusion (30 min coronary occlusion and 120 min reperfusion), iii) preconditioning (3x5 min coronary occlusion) followed by ischemia/reperfusion, or iv) ischemia/reperfusion with postconditioning (6x10s global ischemia/reperfusion at the onset of reperfusion, respectively. Infarct size was significantly reduced by both interventions. Out of 350 different microRNAs assessed by microarray analysis, 147-160 showed detectable expression levels. As compared to microRNA alterations induced by ischemia/reperfusion vs. time-matched non-ischemic controls, 5 microRNAs were significantly affected by both pre- and postconditioning (microRNA-125b*, 139-3p, 320, 532-3p, 188), 4 microRNAs by preconditioning (microRNA-487b, 139-5p, 192, 212), and 9 by postconditioning (microRNA-1, let-7i, let-7e, let7b, 181a, 208, 328, 335, 503), respectively. Expression of randomly selected microRNAs was validated by QRT-PCR. By a systematic comparison of the direction of microRNA expression changes in all groups, we identified microRNAs, specific mimics or antagomiRs of which may have pre- and postconditioning-like cardioprotective effect (protectomiRs). Transfection of selected protectomiRs (mimics of microRNA-139-5p, -125b*, let-7b, and inhibitor of microRNA-487b) into cardiac myocytes subjected to simulated ischemia/reperfusion showed significant cytoprotective effect. This is the first demonstration that ischemia/reperfusion-induced microRNA expression profile is significantly influenced by both pre- and postconditioning, which shows the involvement of microRNAs in cardioprotective signaling. Moreover, by analysis of microRNA expression patterns in cardioprotection by pre- and postconditioning, specific protectomiRs can be revealed as potential therapeutic tools treating ischemia/reperfusion injury.
dc.relation.ispartof urn:issn:0363-6135
dc.title MicroRNAs associated with ischemia/reperfusion injury and cardioprotection by ischemic pre- and postconditioning: ProtectomiRs
dc.type Journal Article
dc.date.updated 2018-08-27T17:01:06Z
dc.language.rfc3066 en
dc.identifier.mtmt 2595524
dc.identifier.wos 000339173600012
dc.identifier.pubmed 24858849
dc.contributor.department SE/AOK/I/Farmakológiai és Farmakoterápiás Intézet
dc.contributor.institution Semmelweis Egyetem
dc.mtmt.swordnote T. Csont and P. Ferdinandy contributed equally to this work.


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