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dc.contributor.author Komlósi, Zsolt
dc.contributor.author Kovacs, N
dc.contributor.author van de Veen, W
dc.contributor.author Kirsch, AI
dc.contributor.author Fahrner, HB
dc.contributor.author Wawrzyniak, M
dc.contributor.author Rebane, A
dc.contributor.author Stanic, B
dc.contributor.author Palomares, O
dc.contributor.author Ruckert, B
dc.contributor.author Menz, G
dc.contributor.author Akdis, M
dc.contributor.author Losonczy, György
dc.contributor.author Akdis, CA
dc.date.accessioned 2020-03-31T11:58:47Z
dc.date.available 2020-03-31T11:58:47Z
dc.date.issued 2018
dc.identifier.citation journalVolume=142;journalIssueNumber=1;journalTitle=JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY;pagerange=178-194.e11;journalAbbreviatedTitle=J ALLERGY CLIN IMMUN;
dc.identifier.uri http://repo.lib.semmelweis.hu//handle/123456789/7797
dc.identifier.uri doi:10.1016/j.jaci.2017.07.046
dc.description.abstract BACKGROUND: Type 3 innate lymphoid cells (ILC3s) are involved in maintenance of mucosal homeostasis; however, their role in immunoregulation has been unknown. Immature transitional regulatory B (itBreg) cells are innate-like B cells with immunosuppressive properties, and the in vivo mechanisms by which they are induced have not been fully clarified. OBJECTIVE: We aimed to investigate the ILC3-B-cell interaction that probably takes place in human tonsils. METHODS: ILC3s were isolated from peripheral blood and palatine tonsils, expanded, and cocultured with naive B cells. Tonsillar ILC3s and regulatory B cells were visualized with immunofluorescence histology. ILC3 frequencies were measured in tonsil tissue of allergic and nonallergic patients and in peripheral blood of allergic asthmatic patients and healthy control subjects. RESULTS: A mutually beneficial relationship was revealed between ILC3s and B cells: ILC3s induced IL-15 production in B cells through B cell-activating factor receptor, whereas IL-15, a potent growth factor for ILC3s, induced CD40 ligand (CD40L) expression on circulating and tonsillar ILC3s. IL-15-activated CD40L(+) ILC3s helped B-cell survival, proliferation, and differentiation of IL-10-secreting, PD-L1-expressing functional itBreg cells in a CD40L- and B cell-activating factor receptor-dependent manner. ILC3s and regulatory B cells were in close connection with each other in palatine tonsils. ILC3 frequency was reduced in tonsil tissue of allergic patients and in peripheral blood of allergic asthmatic patients. CONCLUSION: Human CD40L(+) ILC3s provide innate B-cell help and are involved in an innate immunoregulatory mechanism through induction of itBreg cell differentiation, which takes place in palatine tonsils in vivo. This mechanism, which can contribute to maintenance of immune tolerance, becomes insufficient in allergic diseases.
dc.format.extent 178-194.e11
dc.relation.ispartof urn:issn:0091-6749
dc.title Human CD40 ligand-expressing type 3 innate lymphoid cells induce IL-10-producing immature transitional regulatory B cells
dc.type Journal Article
dc.date.updated 2019-09-19T12:31:38Z
dc.language.rfc3066 en
dc.rights.holder NULL
dc.identifier.mtmt 3329077
dc.identifier.wos 000437837500022
dc.identifier.pubmed 28939410
dc.contributor.department SE/AOK/K/Pulmonológiai Klinika
dc.contributor.institution Semmelweis Egyetem


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