Alsó végtagi ischaemiás-reperfusiós károsodás hosszú távú tüdőszövődményeinek megelőzése posztkondicionálással [Postconditioning can reduce long-term lung injury after lower limb ischemia-reperfusion]

Abstract

Introduction: Operation on the infrarenal aorta could cause ischemic-reperfusion (IR) injury in local tissues and remote organs (e.g. the lung). Objectives: Our aim was to reduce long-term lung damage, after lower limb IR with postconditioning. Materials and methods: Male Wistar rats underwent 180 minutes of bilateral lower limb ischemia. Animals were divided into three groups: Sham-operated, IR, Postconditioned (PostC) and further to two subgroups according to reperfusion time: 24 h and 72 h. Serum free radical and IL-6 levels, histological changes, Wet/Dry (W/D) ratio, tissue myeloperoxidase (MPO) activity and Hsp72 levels were investigated. Results: Postconditioning can reduce histological changes in the lung. Free radical levels are significantly lower in PostC groups than in IR groups (42.9 +/- 8.0 vs. 6.4 +/- 3.4; 27.3 +/- 4.4 vs. 8.3 +/- 4.0 RLU%; p < 0.05). IL-6 level (238.4 +/- 31.1 vs. 209.1 +/- 18.8; 190.0 +/- 8.8 vs. 187.0 +/- 14.9 pg/ml) and Hsp72 expression did not show any significant difference. Compared to the IR group, lung MPO activity did not change in the PostC groups. W/D ratio in PostC groups is significantly lower at all measured time-points (68% vs. 65%; 72% vs. 68%; p < 0.05). Conclusion: Postconditioning may reduce long-term damages of the lung after lower limb ischemic-reperfusion injury.