Show simple item record Levine, Martin Collins, Lindsay M Lohinai, Zsolt 2022-05-20T07:32:41Z 2022-05-20T07:32:41Z 2021
dc.identifier 85097231987
dc.identifier.citation journalVolume=104;pagination=103533, pages: 6;journalTitle=JOURNAL OF DENTISTRY;journalAbbreviatedTitle=J DENTISTRY;
dc.identifier.uri doi:10.1016/j.jdent.2020.103533
dc.description.abstract Objectives Dentifrices containing zinc reduce gingival inflammation and bleeding better than control dentifrices (no zinc). How zinc might work is not understood. We have shown that lysine decarboxylase (LdcE), an enzyme from Eikenella corrodens, converts lysine to cadaverine in dental biofilms. The lack of lysine impairs the dentally attached cell barrier to biofilm, causing biofilm products to leak into junctional epithelium and stimulate inflammation. In year-old beagle dogs, immunization with LdcE, induces antibodies that inhibit LdcE activity and retard gingivitis development. We therefore examined whether a zinc-mediated loss of LdcE activity could explain the beneficial effect of zinc dentifrices. Methods We grew E. corrodens in modified tryptic soy broth with or without zinc chloride, and extracted LdcE from the cell surface using a Potter Elvehjem homogenizer. Results Up to 0.96 mM zinc chloride in the bacterial growth medium did not change cell yield, but reduced the extracted protein content by 41 % (R2 = 0.27, p < 0.05) and LdcE activity/mg extracted protein by 85 % (R2 = 0.90, p < 0.001). In extracts from cells grown without zinc, 78 times this zinc chloride concentration (73 mM) was required to reduce LdcE activity by 75 %. Conclusions Zinc ions inhibit the production of protein with LdcE activity at E. corrodens cell surfaces. The zinc ions may attach to cysteine residues that are unique to the N-terminal region of LdcE by interfering with the non-covalent polypeptide assembly that produces enzyme activity. Clinical significance Zinc ion-mediated inhibition of LdcE assembly may provide a rationale for the improved control of gingival inflammation by zinc dentifrices.
dc.relation.ispartof urn:issn:0300-5712
dc.title Zinc chloride inhibits lysine decarboxylase production from Eikenella corrodens in vitro and its therapeutic implications
dc.type Journal Article 2022-05-19T18:53:11Z
dc.language.rfc3066 en
dc.rights.holder NULL
dc.identifier.mtmt 31712141
dc.identifier.wos 000619736100013
dc.identifier.pubmed 33189800
dc.contributor.department SE/FOK/Konzerváló Fogászati Klinika
dc.contributor.institution Semmelweis Egyetem

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