Show simple item record Markó Lóránt Paragh György Ugocsai Péter Boettcher A Vogt T Schling P Balogh Attila Tarabin V Orsó E Wikonkál Norbert Mandl József Remenyik Éva Schmitz G 2015-01-14T12:04:21Z 2015-01-14T12:04:21Z 2012
dc.identifier 84866001845
dc.identifier.citation pagination=79-88; journalVolume=116; journalIssueNumber=5; journalTitle=JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY;
dc.identifier.uri doi:10.1016/j.jphotobiol.2012.06.007
dc.description.abstract Many ATP binding cassette (ABC) transporters are important regulators of lipid homeostasis and have been implicated in keratinocyte lipid transport. Ultraviolet (UV) light exposure is a known epidermal stressor, which amongst other effects causes lipid alterations and defective lamellar body biogenesis. To elucidate the background of these lipid changes we studied the effect of UVB light on ABC transporter expression. The effect of UVB treatment on the levels of 47 known human ABC transporter mRNAs was analyzed in normal human epidermal keratinocytes. Immunoblots and promoter assays were carried out for ABCA1 and ABCG1. The mRNA levels of cholesterol transport regulators ABCA1 and ABCG1 were markedly downregulated by UVB, parallel to the lamellar ichthyosis related glucosylceramide transporter ABCA12 and the suspected sphingosine-1-phosphate and cholesterol sulfate transporter ABCC1. The long but not the short alternative splice variant of the ABCF2 was found to be markedly upregulated rapidly after UVB irradiation. Immunoblot confirmed ABCA1 and ABCG1 protein downregulation, and luciferase assays showed suppression of their promoters by UVB. These proteins mostly transport lipids, which account for the integrity of the epidermal barrier; therefore our findings on the UVB regulation of ABC transporters may explain the appearance of barrier dysfunction after UVB exposure. © 2012 Elsevier B.V. All rights reserved.
dc.relation.ispartof urn:issn:1011-1344
dc.title Keratinocyte ATP binding cassette transporter expression is regulated by ultraviolet light
dc.type Journal Article 2015-01-08T09:17:25Z
dc.language.rfc3066 en
dc.identifier.mtmt 2096088
dc.identifier.wos 000309991000011
dc.identifier.pubmed 22982209
dc.contributor.department SE/ÁOK/K/Bőr-, Nemikórtani és Bőronkológiai Klinika
dc.contributor.department SE/ÁOK/I/Orvosi Vegytani, Molekuláris Biológiai és Patobiokémiai Intézet
dc.contributor.institution Semmelweis Egyetem

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