Show simple item record Csörgőné Szabó, Szilvia Xu Y, Romero R, Fule T, Karaszi K, Krenács, Tibor Papp, Zoltán Kovalszky, Ilona Than, Nándor Gábor 2015-02-16T11:11:51Z 2015-02-16T11:11:51Z 2013
dc.identifier 84884146687
dc.identifier.citation pagination=445-458; journalVolume=463; journalIssueNumber=3; journalTitle=VIRCHOWS ARCHIV-AN INTERNATIONAL JOURNAL OF PATHOLOGY;
dc.identifier.uri doi:10.1007/s00428-013-1426-0
dc.description.abstract Preeclampsia is characterized by maternal systemic anti-angiogenic and pro-inflammatory states. Syndecan-1 is a cell surface proteoglycan expressed by the syncytiotrophoblast, which plays an important role in angiogenesis and resolution of inflammation. Our aim was to examine placental syndecan-1 expression in preeclampsia with or without hemolysis, elevated liver enzymes, and low platelet count (HELLP) syndrome. Placentas were obtained from women in the following groups: (1) late-onset preeclampsia (n = 8); (2) early-onset preeclampsia without (n = 7) and (3) with HELLP syndrome (n = 8); (4) preterm controls (n = 5); and (5) term controls (n = 9). Tissue microarrays (TMAs) were constructed from paraffin-embedded placentas. TMA slides were immunostained for syndecan-1 and evaluated using microscopy, virtual microscopy, and semi-automated image analysis. Maternal sera from patients with preeclampsia (n = 49) and controls (n = 32) were immunoassayed for syndecan-1. BeWo cells were treated with Forskolin or Latrunculin B or kept in ischemic conditions. SDC1 expression and syndecan-1 production were investigated with qRT-PCR, confocal microscopy, and immunoassays. Syndecan-1 was localized to the syncytiotrophoblast apical membrane in normal placentas. Syndecan-1 immunoscores were higher in late-onset preeclampsia (p = 0.0001) and early-onset preeclampsia with or without HELLP syndrome (p = 0.02 for both) than in controls. Maternal serum syndecan-1 concentration was lower in preeclampsia (median, 673 ng/ml; interquartile range, 459-1,161 ng/ml) than in controls (1,158 ng/ml; 622-1,480 ng/ml). SDC1 expression and syndecan-1 immunostainings in BeWo cells and syndecan-1 concentrations in supernatants increased during cell differentiation. Disruption of the actin cytoskeleton with Latrunculin B decreased syndecan-1 release, while ischemic conditions increased it. Syncytiotrophoblastic syndecan-1 expression depends on the differentiation of villous trophoblasts, and trophoblastic syndecan-1 release is decreased in preeclampsia and HELLP syndrome. This phenomenon may be related to the disturbed syncytiotrophoblastic cortical actin cytoskeleton and associated with maternal anti-angiogenic and pro-inflammatory states in these syndromes. © 2013 Springer-Verlag Berlin Heidelberg (outside the USA).
dc.relation.ispartof urn:issn:0945-6317
dc.title Changes of placental syndecan-1 expression in preeclampsia and HELLP syndrome
dc.type Journal Article 2015-02-16T11:11:17Z
dc.language.rfc3066 en
dc.identifier.mtmt 2376774
dc.identifier.wos 000323904700010
dc.identifier.pubmed 23807541
dc.contributor.department SE/AOK/I/I. Sz. Patológiai és Kísérleti Rákkutató Intézet
dc.contributor.department SE/ETK/AEI/Morfológiai és Fiziológiai Tanszék
dc.contributor.institution Semmelweis Egyetem

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