dc.contributor.author |
D'Este L |
|
dc.contributor.author |
Kulaksiz H |
|
dc.contributor.author |
Rausch U |
|
dc.contributor.author |
Vaccaro R |
|
dc.contributor.author |
Wenger Tibor |
|
dc.contributor.author |
Tokunaga Y |
|
dc.contributor.author |
Renda TG |
|
dc.contributor.author |
Cetin Y |
|
dc.date.accessioned |
2015-03-09T10:32:29Z |
|
dc.date.available |
2015-03-09T10:32:29Z |
|
dc.date.issued |
2000 |
|
dc.identifier.citation |
pagination=1131-1136;journalVolume=97;journalIssueNumber=3;journalTitle=PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA; |
hu |
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/1418 |
|
dc.description.abstract |
The intestinal peptide guanylin regulates the electrolyte/water transport in the gastrointestinal epithelium by paracrine/luminocrine mechanisms. Because guanylin also circulates in the blood, we investigated the rat hypothalamo-pituitary region for expression and cellular localization of this peptide. Reverse transcriptase-PCR analyses with guanylin-specific primers revealed expression of the peptide in the pars tuberalis and pars distalis of the pituitary. Western blotting analyses in hypophyseal tissue extracts identified the expected 12.5-kDa immunoreactive peptide by using two different region-specific guanylin antisera. Light and electron microscopic immunocytochemistry with the same antisera localized guanylin in "pars tuberalis-specific cells" in the juxtaneural pars tuberalis adjacent to nerve endings and blood vessels of the hypothalamo-pituitary portal system and in gonadotrophic cells within the distal pars tuberalis and ventrolateral part of the pars distalis. The presence and cell-specific localization of guanylin within the hypothalamo-hypophyseal system indicate that this peptide may be specifically involved in paracrine and endocrine regulatory mechanisms. |
hu |
dc.relation.ispartof |
urn:issn:0027-8424 |
|
dc.title |
Expression of guanylin in "pars tuberalis-specific cells" and gonadotrophs of rat adenohypophysis. |
hu |
dc.type |
Journal Article |
hu |
dc.date.updated |
2015-02-16T12:45:38Z |
|
dc.language.rfc3066 |
en |
hu |
dc.identifier.mtmt |
2539507 |
|
dc.identifier.pubmed |
10655496 |
|
dc.contributor.department |
SE/AOK/I/Humánmorfológiai és Fejlődésbiológiai Intézet |
|
dc.contributor.institution |
Semmelweis Egyetem |
|