dc.contributor.author |
Szabó Klaudia |
|
dc.contributor.author |
Szabó Arnold |
|
dc.contributor.author |
Énzsöly Anna |
|
dc.contributor.author |
Szél Ágoston |
|
dc.contributor.author |
Lukáts Ákos |
|
dc.date.accessioned |
2015-09-02T09:37:13Z |
|
dc.date.available |
2015-09-02T09:37:13Z |
|
dc.date.issued |
2014 |
|
dc.identifier |
84898913231 |
|
dc.identifier.citation |
pagination=49-63;
journalVolume=356;
journalIssueNumber=1;
journalTitle=CELL AND TISSUE RESEARCH; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/1535 |
|
dc.identifier.uri |
doi:10.1007/s00441-013-1788-2 |
|
dc.description.abstract |
During the first postnatal weeks of the developing rodent
retina, rhodopsin can be detected in a number of neuron-like
cells in the inner retina. In the present study, we aim to
characterize the morphology, number and staining characteristics
of this peculiar population. Misplaced rhodopsin-positive cells
(MRCs) were analyzed on retinas of four rodent species, labeled
with various rhodopsin-specific antibodies. To investigate their
possible relation with non-photoreceptor cells, sections were
double-stained against distinct retinal cell types and proteins
of the phototransduction cascade. The possibility of synapse
formation and apoptosis were also investigated. In all species
studied, misplaced cells comprised a few percent of all
rhodopsin-positive elements. This ratio declined from the end
of the second week and MRCs disappeared nearly completely from
the retina by P24. MRCs resembled resident neurons of the inner
retina, while outer segment-like processes were seen only
rarely. MRCs expressed no other photopigment types and showed
no colocalization with any of the bipolar, horizontal, amacrine
and ganglion cell markers used. While all MRCs colabeled for
arrestin and recoverin, other proteins of the phototransduction
cascade were only detectable in a minority of the population.
Only a few MRCs were shown to form synaptic-like endings. Our
results showed that, during development, some rhodopsin-
expressing cells are displaced to the inner retinal layers.
Although most MRCs lack morphological features of
photoreceptors, they contain some but not all, elements of the
phototransduction cascade, indicating that they are most
probably misplaced rods that failed to complete differentiation
and integrate into the photoreceptor mosaic. |
|
dc.relation.ispartof |
urn:issn:0302-766X |
|
dc.title |
Immunocytochemical analysis of misplaced rhodopsin-positive cells in the developing rodent retina. |
|
dc.type |
Journal Article |
|
dc.date.updated |
2015-03-10T10:24:34Z |
|
dc.language.rfc3066 |
en |
|
dc.identifier.mtmt |
2535952 |
|
dc.identifier.wos |
000334175100006 |
|
dc.identifier.pubmed |
24496510 |
|
dc.contributor.department |
SE/AOK/I/Humánmorfológiai és Fejlődésbiológiai Intézet |
|
dc.contributor.department |
SE/AOK/K/Szemészeti Klinika |
|
dc.contributor.institution |
Semmelweis Egyetem |
|