dc.contributor.author |
Reed DM |
|
dc.contributor.author |
Földes, Gábor |
|
dc.contributor.author |
Gatheral T |
|
dc.contributor.author |
Paschalaki KE |
|
dc.contributor.author |
Lendvai Z |
|
dc.contributor.author |
Bagyura, Zsolt |
|
dc.contributor.author |
Németh, Tamás |
|
dc.contributor.author |
Skopál, Judit |
|
dc.contributor.author |
Merkely, Béla Péter |
|
dc.contributor.author |
Telcian AG |
|
dc.contributor.author |
Gogsadze L |
|
dc.contributor.author |
Edwards MR |
|
dc.contributor.author |
Gough PJ |
|
dc.contributor.author |
Bertin J |
|
dc.contributor.author |
Johnston SL |
|
dc.contributor.author |
Harding SE |
|
dc.contributor.author |
Mitchell JA |
|
dc.date.accessioned |
2015-05-14T14:19:08Z |
|
dc.date.available |
2015-05-14T14:19:08Z |
|
dc.date.issued |
2014 |
|
dc.identifier.citation |
pagination=e91119;
journalVolume=9;
journalIssueNumber=4;
journalTitle=PLOS ONE; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/1544 |
|
dc.identifier.uri |
doi:10.1371/journal.pone.0091119 |
|
dc.description.abstract |
Human embryonic stem cell-derived endothelial cells (hESC-EC), as well as other stem cell derived endothelial cells, have a range of applications in cardiovascular research and disease treatment. Endothelial cells sense Gram-negative bacteria via the pattern recognition receptors (PRR) Toll-like receptor (TLR)-4 and nucleotide-binding oligomerisation domain-containing protein (NOD)-1. These pathways are important in terms of sensing infection, but TLR4 is also associated with vascular inflammation and atherosclerosis. Here, we have compared TLR4 and NOD1 responses in hESC-EC with those of endothelial cells derived from other stem cells and with human umbilical vein endothelial cells (HUVEC). HUVEC, endothelial cells derived from blood progenitors (blood outgrowth endothelial cells; BOEC), and from induced pluripotent stem cells all displayed both a TLR4 and NOD1 response. However, hESC-EC had no TLR4 function, but did have functional NOD1 receptors. In vivo conditioning in nude rats did not confer TLR4 expression in hESC-EC. Despite having no TLR4 function, hESC-EC sensed Gram-negative bacteria, a response that was found to be mediated by NOD1 and the associated RIP2 signalling pathways. Thus, hESC-EC are TLR4 deficient but respond to bacteria via NOD1. This data suggests that hESC-EC may be protected from unwanted TLR4-mediated vascular inflammation, thus offering a potential therapeutic advantage. |
|
dc.relation.ispartof |
urn:issn:1932-6203 |
|
dc.title |
Pathogen Sensing Pathways in Human Embryonic Stem Cell Derived-Endothelial Cells: Role of NOD1 Receptors. |
|
dc.type |
Journal Article |
|
dc.date.updated |
2015-03-11T08:47:15Z |
|
dc.language.rfc3066 |
en |
|
dc.identifier.mtmt |
2583732 |
|
dc.identifier.pubmed |
24690886 |
|
dc.contributor.department |
SE/AOK/K/Kardiológia Központ - Kardiológiai Tanszék |
|
dc.contributor.institution |
Semmelweis Egyetem |
|