dc.contributor.author |
Weaver D |
|
dc.contributor.author |
Eisner V |
|
dc.contributor.author |
Liu X |
|
dc.contributor.author |
Várnai Péter |
|
dc.contributor.author |
Hunyady László |
|
dc.contributor.author |
Gross A |
|
dc.contributor.author |
Hajnóczky György |
|
dc.date.accessioned |
2015-04-08T11:31:55Z |
|
dc.date.available |
2015-04-08T11:31:55Z |
|
dc.date.issued |
2014 |
|
dc.identifier |
84901914053 |
|
dc.identifier.citation |
pagination=870-878;
journalVolume=54;
journalIssueNumber=5;
journalTitle=MOLECULAR CELL; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/1668 |
|
dc.identifier.uri |
doi:10.1016/j.molcel.2014.03.048 |
|
dc.description.abstract |
Cells deficient in mitochondrial fusion have been shown to have defects linked to the exchange of inner membrane and matrix components. Because outer-mitochondrial membrane (OMM) constituents insert directly from the cytoplasm, a role for fusion in their intermitochondrial transfer was unanticipated. Here, we show that fibroblasts lacking the GTPases responsible for OMM fusion, mitofusins 1 and 2 (MFN1 and MFN2), display more heterogeneous distribution of OMM proteins. Proteins with different modes of OMM association display varying degrees of heterogeneity in Mfn1/2-/- cells and different kinetics of transfer during fusion in fusion-competent cells. Proapoptotic Bak exhibits marked heterogeneity, which is normalized upon expression of MFN2. Bak is critical for Bid-induced OMM permeabilization and cytochrome c release, and Mfn1/2-/- cells show dysregulation of Bid-dependent apoptotic signaling. Bid sensitivity of Bak-deficient mitochondria is regained upon fusion with Bak-containing mitochondria. Thus, OMM protein distribution depends on mitochondrial fusion and is a locus of apoptotic dysfunction in conditions of fusion deficiency. © 2014 Elsevier Inc. All rights reserved. |
|
dc.relation.ispartof |
urn:issn:1097-2765 |
|
dc.title |
Distribution and Apoptotic Function of Outer Membrane Proteins Depend on Mitochondrial Fusion |
|
dc.type |
Journal Article |
|
dc.date.updated |
2015-04-08T11:30:37Z |
|
dc.language.rfc3066 |
en |
|
dc.identifier.mtmt |
2599405 |
|
dc.identifier.wos |
000340626100015 |
|
dc.identifier.pubmed |
24813948 |
|
dc.contributor.department |
SE/AOK/I/Élettani Intézet |
|
dc.contributor.institution |
Semmelweis Egyetem |
|