dc.contributor.author |
Dudás József |
|
dc.contributor.author |
Fullár Alexandra |
|
dc.contributor.author |
Angela Romani |
|
dc.contributor.author |
Christian Pritz |
|
dc.contributor.author |
Kovalszky Ilona |
|
dc.contributor.author |
Volker Hans Schartinger |
|
dc.contributor.author |
Georg Mathias Sprinzl |
|
dc.contributor.author |
Herbert Riechelmann |
|
dc.date.accessioned |
2016-01-06T11:42:04Z |
|
dc.date.available |
2016-01-06T11:42:04Z |
|
dc.date.issued |
2013 |
|
dc.identifier.citation |
pagination=800-809;journalVolume=319;journalIssueNumber=6;journalTitle=EXPERIMENTAL CELL RESEARCH; |
hu |
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/1669 |
|
dc.identifier.uri |
doi:10.1016/j.yexcr.2012.12.001 |
|
dc.description.abstract |
Co-culture of periodontal ligament fibroblasts (PDLs) and SCC-25 oral squamous carcinoma cells (OSCC) results in conversion of PDLs into carcinoma-associated fibroblasts (CAFs) and induces epithelial-to mesenchymal transition (EMT) of OSCC tumor cells. We hypothesized that Curcumin targets this dynamic mutual interaction between CAFs and tumor cells. Normal and 2muM Curcumin-treated co-culture were performed for 4 days, followed by analysis of tumor cell invasivity, mRNA/protein expression of EMT-markers and mediators, activity measure of matrix metalloproteinase 9 (MMP-9), and western blot analysis of signal transduction in tumor cells and fibroblasts. In Curcumin-treated co-culture, in tumor cells, the levels of nuclear factor kappaB (NFkappaBalpha) and early response kinase (ERK)-decreased, in fibroblasts, integrin alphav protein synthesis decreased compared to corresponding cells in normal co-culture. The signal modulatory changes induced by Curcumin caused decreased release of EMT-mediators in CAFs and reversal of EMT in tumor cells, which was associated with decreased invasion. These data confirm the palliative potential of Curcumin in clinical application. |
hu |
dc.relation.ispartof |
urn:issn:0014-4827 |
|
dc.title |
Curcumin targets fibroblast-tumor cell interactions in oral squamous cell carcinoma. |
hu |
dc.type |
Journal Article |
hu |
dc.date.updated |
2015-04-08T12:44:51Z |
|
dc.language.rfc3066 |
en |
hu |
dc.identifier.mtmt |
2233921 |
|
dc.identifier.wos |
000316374600004 |
|
dc.identifier.pubmed |
23247073 |
|
dc.contributor.department |
SE/AOK/I/I. Sz. Patológiai és Kísérleti Rákkutató Intézet |
|
dc.contributor.institution |
Semmelweis Egyetem |
|