dc.contributor.author |
Meszaros G |
|
dc.contributor.author |
Orbán, Csaba |
|
dc.contributor.author |
Kaposi, Ambrus |
|
dc.contributor.author |
Toldi, Gergely |
|
dc.contributor.author |
Gyarmati B |
|
dc.contributor.author |
Tulassay, Tivadar |
|
dc.contributor.author |
Vásárhelyi, Barna |
|
dc.date.accessioned |
2016-09-27T10:47:38Z |
|
dc.date.available |
2016-09-27T10:47:38Z |
|
dc.date.issued |
2015 |
|
dc.identifier |
84938930070 |
|
dc.identifier.citation |
pagination=216-227;
journalVolume=102;
journalIssueNumber=2;
journalTitle=ACTA PHYSIOLOGICA HUNGARICA; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/2318 |
|
dc.identifier.uri |
doi:10.1556/036.102.2015.2.12 |
|
dc.description.abstract |
Mitochondrial functions have a major impact on T-cell functionality. In this study we characterized whether mitochondrial function in the neonatal T-cells differs from that in the adult T-cells during short T-cell activation. METHODS: We used fow cytometry methods to test mitochondrial mass and to monitor mitochondrial Ca(2+) levels, mitochondrial potential and superoxide generation in parallel with cytoplasmic Ca(2+) levels during phythohaemagglutinine-induced activation of CD4+ and CD8+ T-cells of 12 term neonates and 11 healthy adults. RESULTS: Baseline mitochondrial mass of CD4+ and CD8+ cells was lower in the neonate than in the adult. In comparison with the adult, neonatal resting CD4+ T-cells had lower cytoplasmic Ca(2+) levels and this was associated with normal activation induced Ca(2+)-response. During short-term activation cytoplasmic Ca(2+)-response was lower in neonatal than in adult CD8+ T-cells. Mitochondrial Ca(2+) uptake was increased in CD4+ neonatal T cells while it decreased in CD8+ T-cells. Mitochondrial depolarization was increased in CD4+ and decreased in CD8+ neonatal T-cells compared to adults. Superoxide generation was higher and equal in neonatal CD4+ and CD8+ cells, respectively, compared to the adult ones. CONCLUSION: Our data suggest that neonatal T-cells exhibit marked differences in mitochondrial function and superoxide generation compared to adult T-cells. |
|
dc.relation.ispartof |
urn:issn:0231-424X |
|
dc.title |
Altered mitochondrial response to activation of T-cells in neonate |
|
dc.type |
Journal Article |
|
dc.date.updated |
2015-11-09T11:54:20Z |
|
dc.language.rfc3066 |
en |
|
dc.identifier.mtmt |
2920562 |
|
dc.identifier.wos |
000356627700012 |
|
dc.identifier.pubmed |
26100311 |
|
dc.contributor.department |
SE/AOK/K/I. Sz. Gyermekgyógyászati Klinika |
|
dc.contributor.department |
SE/AOK/K/ISZGYK/MTA-SE Gyermekgyógyászati és Nephrológiai Kutatócsoport |
|
dc.contributor.department |
SE/AOK/I/Laboratóriumi Medicina Intézet |
|
dc.contributor.institution |
Semmelweis Egyetem |
|