Show simple item record Szebényi, Kornélia Füredi, András Kolacsek, Orsolya Csohány, Rózsa Prókai, Ágnes Kis-Petik, Katalin Szabó, Attila Bosze Z Bender B Tóvári, József Enyedi, Ágnes Orbán, Tamás I. Apáti, Ágota Sarkadi, Balázs 2016-12-22T13:21:20Z 2016-12-22T13:21:20Z 2015
dc.identifier.citation pagination=2731-2740; journalVolume=26; journalIssueNumber=11; journalTitle=JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY;
dc.identifier.uri doi:10.1681/ASN.2014070705
dc.description.abstract Intrarenal changes in cytoplasmic calcium levels have a key role in determining pathologic and pharmacologic responses in major kidney diseases. However, cell-specific delivery of calcium-sensitive probes in vivo remains problematic. We generated a transgenic rat stably expressing the green fluorescent protein-calmodulin-based genetically encoded calcium indicator (GCaMP2) predominantly in the kidney proximal tubules. The transposon-based method used allowed the generation of homozygous transgenic rats containing one copy of the transgene per allele with a defined insertion pattern, without genetic or phenotypic alterations. We applied in vitro confocal and in vivo two-photon microscopy to examine basal calcium levels and ligand- and drug-induced alterations in these levels in proximal tubular epithelial cells. Notably, renal ischemia induced a transient increase in cellular calcium, and reperfusion resulted in a secondary calcium load, which was significantly decreased by systemic administration of specific blockers of the angiotensin receptor and the Na-Ca exchanger. The parallel examination of in vivo cellular calcium dynamics and renal circulation by fluorescent probes opens new possibilities for physiologic and pharmacologic investigations.
dc.relation.ispartof urn:issn:1046-6673
dc.title Visualization of Calcium Dynamics in Kidney Proximal Tubules
dc.type Journal Article 2015-11-17T12:17:00Z
dc.language.rfc3066 en
dc.identifier.mtmt 2870811
dc.identifier.pubmed 25788535
dc.contributor.department SE/AOK/K/ISZGYK/MTA-SE Gyermekgyógyászati és Nephrológiai Kutatócsoport
dc.contributor.department SE/AOK/K/I. Sz. Gyermekgyógyászati Klinika
dc.contributor.department SE/AOK/I/Biofizikai és Sugárbiológiai Intézet
dc.contributor.department SE/AOK/I/II. Sz. Patológiai Intézet
dc.contributor.institution Semmelweis Egyetem

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