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dc.contributor.author Szebényi, Kornélia
dc.contributor.author Füredi, András
dc.contributor.author Kolacsek, Orsolya
dc.contributor.author Csohány, Rózsa
dc.contributor.author Prókai, Ágnes
dc.contributor.author Kis-Petik, Katalin
dc.contributor.author Szabó, Attila
dc.contributor.author Bosze Z
dc.contributor.author Bender B
dc.contributor.author Tóvári, József
dc.contributor.author Enyedi, Ágnes
dc.contributor.author Orbán, Tamás I.
dc.contributor.author Apáti, Ágota
dc.contributor.author Sarkadi, Balázs
dc.date.accessioned 2016-12-22T13:21:20Z
dc.date.available 2016-12-22T13:21:20Z
dc.date.issued 2015
dc.identifier.citation pagination=2731-2740; journalVolume=26; journalIssueNumber=11; journalTitle=JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY;
dc.identifier.uri http://repo.lib.semmelweis.hu//handle/123456789/2358
dc.identifier.uri doi:10.1681/ASN.2014070705
dc.description.abstract Intrarenal changes in cytoplasmic calcium levels have a key role in determining pathologic and pharmacologic responses in major kidney diseases. However, cell-specific delivery of calcium-sensitive probes in vivo remains problematic. We generated a transgenic rat stably expressing the green fluorescent protein-calmodulin-based genetically encoded calcium indicator (GCaMP2) predominantly in the kidney proximal tubules. The transposon-based method used allowed the generation of homozygous transgenic rats containing one copy of the transgene per allele with a defined insertion pattern, without genetic or phenotypic alterations. We applied in vitro confocal and in vivo two-photon microscopy to examine basal calcium levels and ligand- and drug-induced alterations in these levels in proximal tubular epithelial cells. Notably, renal ischemia induced a transient increase in cellular calcium, and reperfusion resulted in a secondary calcium load, which was significantly decreased by systemic administration of specific blockers of the angiotensin receptor and the Na-Ca exchanger. The parallel examination of in vivo cellular calcium dynamics and renal circulation by fluorescent probes opens new possibilities for physiologic and pharmacologic investigations.
dc.relation.ispartof urn:issn:1046-6673
dc.title Visualization of Calcium Dynamics in Kidney Proximal Tubules
dc.type Journal Article
dc.date.updated 2015-11-17T12:17:00Z
dc.language.rfc3066 en
dc.identifier.mtmt 2870811
dc.identifier.pubmed 25788535
dc.contributor.department SE/AOK/K/ISZGYK/MTA-SE Gyermekgyógyászati és Nephrológiai Kutatócsoport
dc.contributor.department SE/AOK/K/I. Sz. Gyermekgyógyászati Klinika
dc.contributor.department SE/AOK/I/Biofizikai és Sugárbiológiai Intézet
dc.contributor.department SE/AOK/I/II. Sz. Patológiai Intézet
dc.contributor.institution Semmelweis Egyetem


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