dc.contributor.author |
Denes L |
|
dc.contributor.author |
Bori, Zoltán |
|
dc.contributor.author |
Csonka E |
|
dc.contributor.author |
Entz, László |
|
dc.contributor.author |
Nagy, Zoltán |
|
dc.date.accessioned |
2016-10-13T09:07:27Z |
|
dc.date.available |
2016-10-13T09:07:27Z |
|
dc.date.issued |
2008 |
|
dc.identifier.citation |
pagination=1022-1024;
journalVolume=39;
journalIssueNumber=3;
journalTitle=STROKE; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/2626 |
|
dc.identifier.uri |
doi:10.1161/STROKEAHA.107.495754 |
|
dc.description.abstract |
BACKGROUND AND PURPOSE: Myointimal hyperplasia (MIH) cells are related to permanent upregulated proliferation as tumor-like cells. The aim of this study is to assess whether treatment of cells after hypoxia by Iroxanadine heat-shock protein (HSP-coinducer) predicts recovery through cell proliferation. METHODS: Vascular smooth muscle cells (VSMC) and brain capillary endothelial cells (HBEC) were isolated from human origin and MIH-cells from early carotid restenosis after surgery. Cell proliferation was quantified by bromuridine (BrdU) incorporation after hypoxia/reoxygenation. HSP72 and cyclin-dependent kinase (CDKN1A) mRNA expression was assessed by reverse transcription-polymerase chain reaction (PCR) and cell cycle distribution by flow cytometry (FACS) analysis. RESULTS: After hypoxia/reoxygenation, the proliferation of MIH-cells increased, whereas endothelial cells decreased (MIH: 0.266+/-0.016 versus 0.336+/-0.024; P<0.05; HBEC: 1.249+/-0.10 versus 0.878+/-0.11; P<0.05). Whereas augmented proliferation of MIH-cells was reduced (40% to 45%) by HSP-coinducer, diminished HBEC proliferation increased (46.2%). Stress-activated-protein-kinase (SAPK)p38-dependent cell cycle redistribution was generated by an increase in HSP72 and CDKN1A mRNA levels in MIH-cells. CONCLUSIONS: The 2 key players of early restenosis (MIH, EC) were oppositely regulated and correspondingly after treatment by HSP-coinducer reverse recovered. Drug candidate may have therapeutic potential in (re)restenosis. |
|
dc.relation.ispartof |
urn:issn:0039-2499 |
|
dc.title |
Reverse regulation of endothelial cells and myointimal hyperplasia on cell proliferation by a heatshock protein-coinducer after hypoxia. |
|
dc.type |
Journal Article |
|
dc.date.updated |
2015-11-24T10:41:23Z |
|
dc.language.rfc3066 |
en |
|
dc.identifier.mtmt |
1625369 |
|
dc.identifier.wos |
000253542800043 |
|
dc.identifier.pubmed |
18239173 |
|
dc.contributor.department |
SE/AOK/K/Érsebészeti Klinika [2011.12.31] |
|
dc.contributor.institution |
Semmelweis Egyetem |
|