dc.contributor.author |
Bányai, Péter |
|
dc.contributor.author |
Kuzovkina IN, |
|
dc.contributor.author |
Kursinszki, László |
|
dc.contributor.author |
Szőke, Éva |
|
dc.date.accessioned |
2016-02-12T18:31:18Z |
|
dc.date.available |
2016-02-12T18:31:18Z |
|
dc.date.issued |
2006 |
|
dc.identifier.citation |
pagination=111-114;
journalVolume=63;
journalIssueNumber=13;
journalTitle=CHROMATOGRAPHIA; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/3055 |
|
dc.identifier.uri |
doi:10.1365/s10337-006-0792-z |
|
dc.description.abstract |
We have determined the quantities of the anthraquinones alizarin and
purpurin, with especial regard to their effective antigenotoxic
activity, in genetically transformed hairy root cultures of Rubia
tinctorum L. Hairy roots were cultured on solid and in liquid Gamborg
B5 and 1/2 NMS media in a shaking cabinet and in a bioreactor.
Methanolic extracts of lyophilized hairy roots were hydrolysed, and
then purified by solid phase extraction with good recovery. A new HPLC
method was developed for the determination of alizarin and purpurin.
The analysis was performed on a Luna C8 RP column using a 45:55 (v/v)
mixture of acetonitrile:20 mM ammonium formate-formic acid buffer (pH
3.00) as eluent. Peaks were identified by addition of standards and by
diode-array detection. External standardization allowed the
determination of alizarin and purpurin with good sensitivity and
reliability. The maximum purpurin content was observed in cultures
cultivated on solid B5 medium (5.94 mg g(-1)). The highest alizarin
content was measured in cultures cultivated on solid 1/2 NMS medium
(2.14 mg g(-1)). |
|
dc.relation.ispartof |
urn:issn:0009-5893 |
|
dc.title |
HPLC analysis of alizarin and purpurin produced by Rubia tinctorum L. hairy root cultures |
|
dc.type |
Journal Article |
|
dc.date.updated |
2016-02-01T13:29:34Z |
|
dc.language.rfc3066 |
en |
|
dc.identifier.mtmt |
1131325 |
|
dc.identifier.wos |
000239016200018 |
|
dc.contributor.department |
SE/GYTK/Farmakognózia Intézet |
|
dc.contributor.institution |
Semmelweis Egyetem |
|