dc.contributor.author |
Langeslay DJ, |
|
dc.contributor.author |
Young RP, |
|
dc.contributor.author |
Béni, Szabolcs |
|
dc.contributor.author |
Beecher CN, |
|
dc.contributor.author |
Mueller LJ, |
|
dc.date.accessioned |
2016-02-18T12:17:37Z |
|
dc.date.available |
2016-02-18T12:17:37Z |
|
dc.date.issued |
2012 |
|
dc.identifier |
84864419337 |
|
dc.identifier.citation |
pagination=1173-1182;
journalVolume=22;
journalIssueNumber=9;
journalTitle=GLYCOBIOLOGY; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/3123 |
|
dc.identifier.uri |
doi:10.1093/glycob/cws085 |
|
dc.description.abstract |
Sulfamate groups (NHSO3 -) are important structural elements in the glycosaminoglycans (GAGs) heparin and heparan sulfate (HS). In this work, proton nuclear magnetic resonance (NMR) line-shape analysis is used to explore the solvent exchange properties of the sulfamate NH groups within heparin-related mono-, di-, tetra-and pentasaccharides as a function of pH and temperature. The results of these experiments identified a persistent hydrogen bond within the Arixtra (fondaparinux sodium) pentasaccharide between the internal glucosamine sulfamate NH and the adjacent 3-O-sulfo group. This discovery provides new insights into the solution structure of the Arixtra pentasaccharide and suggests that 3-O-sulfation of the heparin N-sulfoglucosamine (GlcNS) residues pre-organize the secondary structure in a way that facilitates binding to antithrombin-III. NMR studies of the GlcNS NH groups can provide important information about heparin structure complementary to that available from NMR spectral analysis of the carbon-bound protons. © 2012 The Author. |
|
dc.relation.ispartof |
urn:issn:0959-6658 |
|
dc.title |
Sulfamate proton solvent exchange in heparin oligosaccharides: Evidence for a persistent hydrogen bond in the antithrombin-binding pentasaccharide Arixtra |
|
dc.type |
Journal Article |
|
dc.date.updated |
2016-02-17T09:29:46Z |
|
dc.language.rfc3066 |
en |
|
dc.identifier.mtmt |
2159536 |
|
dc.identifier.wos |
000308057600003 |
|
dc.identifier.pubmed |
22593556 |
|
dc.contributor.department |
SE/GYTK/Gyógyszerészi Kémiai Intézet |
|
dc.contributor.institution |
Semmelweis Egyetem |
|