dc.contributor.author |
Szőke, Éva |
|
dc.contributor.author |
Maday E, |
|
dc.contributor.author |
Kiss SA, |
|
dc.contributor.author |
Sonnewend L, |
|
dc.contributor.author |
Lemberkovics, Éva |
|
dc.date.accessioned |
2016-03-30T09:35:21Z |
|
dc.date.available |
2016-03-30T09:35:21Z |
|
dc.date.issued |
2004 |
|
dc.identifier.citation |
pagination=763S-767S;
journalVolume=23;
journalIssueNumber=6;
journalTitle=JOURNAL OF THE AMERICAN COLLEGE OF NUTRITION; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/3141 |
|
dc.identifier.uri |
doi:10.1080/07315724.2004.10719425 |
|
dc.description.abstract |
Objective: The importance of chamomile (Chamomilla recutita) is widely
known in classical and folk medicine, with the largest group of its
effective substances forming the essential oil (chamazulene,
alpha-bisabolol, trans-beta-farnesene, spathulenol,
cis/trans-en-in-dicycloethers). The increasing need for plant-derived
high quality drugs cannot be provided by their collection in the
wilderness.
Method A: To preserve the genome of Szabadkigyos wild type having high
(-)-alpha-bisabolol content, we used biotechnological methods.
Results: The roots of organized culture contained beta-eudesmol, which
we have identified in the intact roots. Our gas-chromatographic and
mass-spectroscopic studies showed that sterile chamomile cultures
generated the most important terpenoid and polyin compounds
characteristics of the intact plant. We identified berkheyaradulene,
geranyl-isovalerate and cedrol, as new components in these cultures.
Magnesium (Mg) (370 and 740 mg/l MgSO4) has a positive effect on the
growth of organized cultures and also on the quality and quantity of
essential oil production.
Method B: Another possible source of variants is available by the
genetic transformation of organized cultures by infection with
Agrobacterium rhisogenes. With this method, we cultivated chamomile
infected by A4-Y clone and investigated the essential oil production by
hairy root cultures cultivated on solid and liquid MS B-5 media. The
main component of the essential oil of hairy root cultures was
trans-beta-farnesene.
Results: We identified a-selinene, as a new component in these hairy
roots. We studied the growth rate of A4-Y clone on the cited media,
containing MgSO4 concentrations: 0; 185; 370 and 740 mg/l. The cultures
grew most in medium containing 740 mg/l of MgSO4. Essential oil content
was compared from hairy root cultures of different Mg containing media
and measured by GC and GC-MS methods. Mg has a similar effect on hairy
roots as on organized cultures. |
|
dc.relation.ispartof |
urn:issn:0731-5724 |
|
dc.title |
Effect of magnesium on essential oil formation of genetically transformed and non-transformed chamomile cultures |
|
dc.type |
Journal Article |
|
dc.date.updated |
2016-02-26T10:09:48Z |
|
dc.language.rfc3066 |
en |
|
dc.identifier.mtmt |
1131329 |
|
dc.identifier.wos |
000227521500021 |
|
dc.identifier.pubmed |
15637231 |
|
dc.contributor.department |
SE/GYTK/Farmakognózia Intézet |
|
dc.contributor.institution |
Semmelweis Egyetem |
|