dc.contributor.author |
Stangl, Rita |
|
dc.contributor.author |
Szijártó, Attila |
|
dc.contributor.author |
Ónody, Péter Zoltán |
|
dc.contributor.author |
Tamás, Judit |
|
dc.contributor.author |
Tátrai M |
|
dc.contributor.author |
Hegedüs V |
|
dc.contributor.author |
Blázovics, Anna |
|
dc.contributor.author |
Lotz, Gábor |
|
dc.contributor.author |
Kiss, András |
|
dc.contributor.author |
Módis K |
|
dc.contributor.author |
Gerő D |
|
dc.contributor.author |
Szabó Cs |
|
dc.contributor.author |
Kupcsulik, Péter |
|
dc.contributor.author |
Harsányi, László |
|
dc.date.accessioned |
2016-09-09T07:07:07Z |
|
dc.date.available |
2016-09-09T07:07:07Z |
|
dc.date.issued |
2011 |
|
dc.identifier |
79951682208 |
|
dc.identifier.citation |
pagination=95-103;
journalVolume=166;
journalIssueNumber=1;
journalTitle=JOURNAL OF SURGICAL RESEARCH; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/3441 |
|
dc.identifier.uri |
doi:10.1016/j.jss.2009.09.047 |
|
dc.description.abstract |
BACKGROUND: Surgical methods that reduce bleeding during major hepatic resections lead to warm ischemia-reperfusion (I-R) injury of the liver. This is well known to have a considerable impact on the postoperative outcome. Much research work has been done to develop possible protective techniques. We aimed to investigate the effectivity of L-alanyl-L-glutamine dipeptide pretreatment in an animal model of hepatic I-R injury. MATERIALS AND METHODS: Male Wistar rats underwent normothermic, 60min segmental liver ischemia followed by 24h of reperfusion. The animals (n=30) were divided into three experimental groups: sham operated, I-R, and glutamine (Gln) pretreated. Twenty-four h prior to I-R injury, rats in the Gln group received 500mg/kg Dipeptiven infusion as glutamine pretreatment. Hepatic microcirculation during the first hour of reperfusion was monitored by noninvasive laser Doppler flowmeter. After a 24-h reperfusion period, liver tissue was analyzed by histologic and immunohistochemical assessments. Serum necroenzyme and antioxidant levels were measured. RESULTS: In the Gln group, the integral of the reperfusion curve (RA) and the plateau maximum (PM(10)) of the flow graph showed improving tendency (RA: P=0.096; PM(10): P=0.084). Severity of histologic damage was reduced. Serum necroenzymes (ALT: P=0.042, AST: P=0.044) were significantly lower. Chemiluminescent intensity of liver and plasma was significantly decreased (P=0.0003 and P=0.0496). Further spectrophotometric analysis of liver homogenate samples also showed significant improvement of the redox homeostasis. CONCLUSIONS: Our results suggest that L-alanyl-L-glutamine dipeptide pretreatment given 24h prior to I-R injury could be an effective method to reduce liver damage caused by hepatic inflow occlusion. |
|
dc.relation.ispartof |
urn:issn:0022-4804 |
|
dc.title |
Reduction of liver ischemia-reperfusion injury via glutamine pretreatment |
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dc.type |
Journal Article |
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dc.date.updated |
2016-06-08T12:14:21Z |
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dc.language.rfc3066 |
en |
|
dc.identifier.mtmt |
1479113 |
|
dc.identifier.wos |
000288167300027 |
|
dc.identifier.pubmed |
20080263 |
|
dc.contributor.department |
SE/AOK/K/II. Sz. Belgyógyászati Klinika |
|
dc.contributor.department |
SE/AOK/K/I. Sz. Sebészeti Klinika |
|
dc.contributor.department |
SE/AOK/I/II. Sz. Patológiai Intézet |
|
dc.contributor.department |
SE/AOK/I/Klinikai Kísérleti Kutató- és Humán Élettani Intézet |
|
dc.contributor.institution |
Semmelweis Egyetem |
|
dc.mtmt.swordnote |
Stangl R and Szíjártó A contributed equally to this study |
|