Kivonat:
The incidence of thyroid cancers is increasing worldwide. Some
somatic oncogene mutations (BRAF, NRAS, HRAS, KRAS) as well as gene
translocations (RET/PTC, PAX8/PPAR-gamma) have been associated with
the development of thyroid cancer. In our study, we analyzed these
genetic alterations in 394 thyroid tissue samples (197 papillary
carcinomas and 197 healthy). The somatic mutations and
translocations were detected by Light Cycler melting method and
Real-Time Polymerase Chain Reaction techniques, respectively. In
tumorous samples, 86 BRAF (44.2 %), 5 NRAS (3.1 %), 2 HRAS (1.0 %)
and 1 KRAS (0.5 %) mutations were found, as well as 9 RET/PTC1 (4.6
%) and 1 RET/PTC3 (0.5 %) translocations. No genetic alteration was
seen in the non tumorous control thyroid tissues. No correlation
was detected between the genetic variants and the pathological
subtypes of papillary cancer as well as the severity of the
disease. Our results are only partly concordant with the data found
in the literature.