dc.contributor.author |
Szűcs Dániel |
|
dc.contributor.author |
Béres Nóra Judit |
|
dc.contributor.author |
Rokonay Réka |
|
dc.contributor.author |
Boros Kriszta |
|
dc.contributor.author |
Borka Katalin |
|
dc.contributor.author |
Kiss Zoltán |
|
dc.contributor.author |
Arató András |
|
dc.contributor.author |
Szabó Attila J |
|
dc.contributor.author |
Vannay Ádám |
|
dc.contributor.author |
Sziksz Erna |
|
dc.contributor.author |
Bereczki Csaba |
|
dc.contributor.author |
Veres Gábor |
|
dc.date.accessioned |
2016-10-10T15:22:56Z |
|
dc.date.available |
2016-10-10T15:22:56Z |
|
dc.date.issued |
2016 |
|
dc.identifier.citation |
pagination=6027-6035;
journalVolume=22;
journalIssueNumber=26;
journalTitle=WORLD JOURNAL OF GASTROENTEROLOGY; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/3699 |
|
dc.identifier.uri |
doi:10.3748/wjg.v22.i26.6027 |
|
dc.description.abstract |
AIM:
To evaluate the role of microRNA (miR)-146a, -155 and -122 in the duodenal mucosa of pediatric patients with Crohn's disease (CD) and the effect of transforming growth factor-β (TGF-β) on these miRs in duodenal epithelial and fibroblast cells.
METHODS:
Formalin-fixed, paraffin-embedded biopsies derived from the macroscopically inflamed (CD inflamed: n = 10) and intact (CD intact: n = 10) duodenal mucosa of pediatric CD patients and control children (C: n = 10) were examined. Expression of miR-146a, -155 and -122 was determined by real-time polymerase-chain reaction (PCR). The expression of the above miRs was investigated in recombinant human TGF-β (1 nmol/L, 24 h) or vehicle treated small intestinal epithelial cells (CCL-241) and primary duodenal fibroblast cells derived from healthy children as well.
RESULTS:
Expression of miR-146a was significantly higher in the inflamed duodenal mucosa compared to the intact duodenal mucosa of children with CD (CD inflamed: 3.21 ± 0.50 vs CD intact: 0.62 ± 0.26, P ≤ 0.01) and to the control group (CD inflamed: 3.21 ± 0.50 vs C: 1.00 ± 0.33, P ≤ 0.05). The expression of miR-155 was significantly increased in the inflamed region of the duodenum compared to the control group (CD inflamed: 4.87 ± 1.02 vs
CONTROL:
1.00 ± 0.40, P ≤ 0.001). The expression of miR-122 was unchanged in the inflamed or intact mucosa of CD patients compared to controls. TGF-β treatment significantly decreased the expression of miR-155 in small intestinal epithelial cells (TGF-β: 0.7 ± 0.083 vs
CONTROL:
1 ± 0.09, P ≤ 0.05) and also the expression of miR-146a (TGF-β: 0.67 ± 0.04 vs
CONTROL:
1 ± 0.15, P ≤ 0.01) and miR-155 (TGF-β: 0.72 ± 0.09 vs
CONTROL:
1 ± 0.06, P ≤ 0.05) in primary duodenal fibroblasts compared to corresponding vehicle treated controls. TGF-β treatment did not influence the expression of miR-122.
CONCLUSION:
The elevated expression of miR-146a and -155 in the inflamed duodenal mucosa of CD patients suggests the role of these miRs in the pathomechanism of inflammatory bowel disease. Anti-inflammatory TGF-β plays an important role in the regulation of the expression of these miRs. |
|
dc.relation.ispartof |
urn:issn:1007-9327 |
|
dc.title |
Increased duodenal expression of miR-146a and-155 in pediatric Crohn's disease |
|
dc.type |
Journal Article |
|
dc.date.updated |
2016-10-03T12:21:52Z |
|
dc.language.rfc3066 |
en |
|
dc.identifier.mtmt |
3120331 |
|
dc.identifier.wos |
000380769300014 |
|
dc.identifier.pubmed |
27468194 |
|
dc.contributor.department |
SE/AOK/K/I. Sz. Gyermekgyógyászati Klinika |
|
dc.contributor.department |
SE/AOK/II. Sz. Patológiai Intézet |
|
dc.contributor.institution |
Semmelweis Egyetem |
|