Show simple item record Wagner, Zsolt Tábi, Tamás Jakó, Tamás Zachar, Gergely Csillag, András Szökő, Éva 2016-11-10T16:30:27Z 2016-11-10T16:30:27Z 2012
dc.identifier 84870861239
dc.identifier.citation pagination=2363-2368; journalVolume=404; journalIssueNumber=8; journalTitle=ANALYTICAL AND BIOANALYTICAL CHEMISTRY;
dc.identifier.uri doi:10.1007/s00216-012-6384-x
dc.description.abstract Chiral capillary electrophoresis method has been developed to separate aspartate and glutamate enantiomers to investigate the putative neuromodulator function of D-Asp in the central nervous system. To achieve appropriate detection sensitivity fluorescent derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole and laser-induced fluorescence detection was applied. Although, simultaneous baseline separation of the two enantiomer pairs could be achieved by using 3 mM 6-monodeoxy-6-mono(3-hydroxy)propylamino-beta-cyclodextrin (HPA-beta-CD), further improvement of the chemical selectivity was required because of the high excess of L-enantiomers in real samples to be analyzed. The system selectivity was fine-tuned by combination of 8 mM heptakis(2,6-di-O-methyl)-beta-cyclodextrin and 5 mM HPA-beta-CD in order to increase the resolution between aspartate and glutamate enantiomers. The method was validated for biological application. The limits of detection for D-Asp and D-Glu were 17 and 9 nM, respectively, while the limit of quantification for both analytes was 50 nM. This is the lowest quantification limit reported so far for NBD-tagged D-Asp and D-Glu obtained by validated capillary electrophoresis laser-induced fluorescence method. The applicability of the method was demonstrated by analyzing brain samples of 1-day-old chickens. In all the studied brain areas, the D-enantiomer contributed 1-2 % of the total aspartate content, corresponding to 17-45 nmol/g wet tissue.
dc.relation.ispartof urn:issn:1618-2642
dc.title Chiral separation and determination of excitatory amino acids in brain samples by CE-LIF using dual cyclodextrin system.
dc.type Journal Article 2016-11-10T14:43:57Z
dc.language.rfc3066 en
dc.identifier.mtmt 2057442
dc.identifier.wos 000310083900028
dc.identifier.pubmed 22960871
dc.contributor.department SE/AOK/I/Anatómiai, Szövet- és Fejlődéstani Intézet [2015.12.31]
dc.contributor.institution Semmelweis Egyetem

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