dc.contributor.author |
Vannay, Ádám |
|
dc.contributor.author |
Fekete, Andrea |
|
dc.contributor.author |
Ádori, Csaba |
|
dc.contributor.author |
Toth T |
|
dc.contributor.author |
Losonczy, György |
|
dc.contributor.author |
László, Lajos |
|
dc.contributor.author |
Vásárhelyi, Barna |
|
dc.contributor.author |
Tulassay, Tivadar |
|
dc.contributor.author |
Szabó, András |
|
dc.date.accessioned |
2017-06-01T11:27:33Z |
|
dc.date.available |
2017-06-01T11:27:33Z |
|
dc.date.issued |
2004 |
|
dc.identifier |
3543051099 |
|
dc.identifier.citation |
pagination=435-444;
journalVolume=89;
journalIssueNumber=4;
journalTitle=EXPERIMENTAL PHYSIOLOGY; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/4243 |
|
dc.identifier.uri |
doi:10.1113/expphysiol.2004.027516 |
|
dc.description.abstract |
Vascular endothelial growth factor (VEGF) is a potent regulator of angiogenesis and vascular protection. Synthesis of VEGF is induced by hypoxia and different cytokines including interleukin-6 (IL-6) and interleukin-1beta (IL-1beta). However, post-ischaemic alterations of this growth factor in the kidney are incompletely known. To determine VEGF synthesis in renal ischaemia/reperfusion (I/R) injury unilateral warm ischaemia was induced by cross-clamping the left renal pedicle for 55 min followed by 2 and 24 h of reperfusion (T-2 and T-24 kidneys; n = 6 in each group). Sham-operated, non-clamped animals served as controls (n = 6). Renal VEGF, IL-6 and 1L-1beta mRNA expression were determined by reverse transcription-polymerase chain reaction (RT-PCR). VEGF protein level and distribution were determined by Western blot and immunohistochemical analysis. Immunohistochemistry revealed prominent VEGF staining in the outer medulla of control, T-2 and T-24 kidneys. VEGF immunoreactivity accumulated at the basolateral area of tubular epithelial cells in T-2 kidneys, while it was diffuse in control and T-24 kidneys. VEGF protein levels were increased 2- to 3-fold in T-2 and T-24 kidneys (both P < 0.01 versus controls), while VEGF mRNA expression remained unchanged. IL-6 mRNA expression was increased (P < 0.01 versus controls) in T-2 kidneys, while IL-1beta mRNA expression remained unchanged. Increased VEGF protein levels but not mRNA expression suggests that during renal I/R injury VEGF synthesis in kidneys - unlike in other organs - is primarily regulated at a post-transcriptional level. As IL-6 mRNA expression increased simultaneously with VEGF protein levels, the post-ischaemic regulation of IL-6 and VEGF synthesis might be interrelated in rat kidney. |
|
dc.relation.ispartof |
urn:issn:0958-0670 |
|
dc.title |
Divergence of renal vascular endothelial growth factor mRNA expression and protein level in post-ischaemic rat kidneys |
|
dc.type |
Journal Article |
|
dc.date.updated |
2017-04-04T10:41:49Z |
|
dc.language.rfc3066 |
en |
|
dc.identifier.mtmt |
1003326 |
|
dc.identifier.wos |
000222751800014 |
|
dc.identifier.pubmed |
15131073 |
|
dc.contributor.department |
SE/AOK/K/I. Sz. Gyermekgyógyászati Klinika |
|
dc.contributor.department |
SE/AOK/I/II. Sz. Patológiai Intézet |
|
dc.contributor.department |
SE/AOK/K/Pulmonológiai Klinika |
|
dc.contributor.department |
SE/AOK/K/ISZGYK/MTA-SE Gyermekgyógyászati és Nephrológiai Kutatócsoport |
|
dc.contributor.institution |
Semmelweis Egyetem |
|