Show simple item record Móricz, Ágnes Krüzselyi, Dániel Alberti, Ágnes Darcsi, András Horváth, Györgyi Csontos, Péter Béni, Szabolcs Ott, Péter 2018-01-17T08:08:26Z 2018-01-17T08:08:26Z 2017
dc.identifier.citation pagination=266-272; journalVolume=1524; journalTitle=JOURNAL OF CHROMATOGRAPHY A;
dc.identifier.uri doi:10.1016/j.chroma.2017.09.062
dc.description.abstract The antibacterial profiling of Onopordum acanthium L. leaf extract and subsequent targeted identification of active compounds is demonstrated. Thin-layer chromatography (TLC) and off-line overpressured layer chromatography (OPLC) coupled with direct bioautography were utilized for investigation of the extract against eight bacterial strains including two plant and three human pathogens and a soil, a marine and a probiotic human gut bacteria. Antibacterial fractions obtaining infusion-transfusion OPLC were transferred to HPLC-MS/MS analysis that resulted in the characterization of three active compounds and two of them were identified as, linoleic and linolenic acid. OPLC method was adopted to preparative-scale flash chromatography for the isolation of the third active compound, which was identified after a further semi-preparative HPLC purification as the germacranolide sesquiterpene lactone onopordopicrin. Pure onopordopicrin exhibited antibacterial activity that was specified as minimal inhibitory concentration in the liquid phase as well.
dc.relation.ispartof urn:issn:0021-9673
dc.title Layer chromatography-bioassays directed screening and identification of antibacterial compounds from Scotch thistle
dc.type Journal Article 2017-10-02T14:21:23Z
dc.language.rfc3066 en
dc.identifier.mtmt 3273429
dc.contributor.department SE/GYTK/Farmakognózia Intézet
dc.contributor.institution Semmelweis Egyetem

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