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dc.contributor.author Rosero Olivér
dc.contributor.author Ónody Péter Zoltán
dc.contributor.author Kovács Tibor
dc.contributor.author Molnár Dávid
dc.contributor.author Fülöp András
dc.contributor.author Lotz Gábor
dc.contributor.author Harsányi László
dc.contributor.author Szijártó Attila
dc.date.accessioned 2018-10-03T12:46:59Z
dc.date.available 2018-10-03T12:46:59Z
dc.date.issued 2017
dc.identifier.citation pagination=1004-1015; journalVolume=161; journalIssueNumber=4; journalTitle=SURGERY;
dc.identifier.uri http://repo.lib.semmelweis.hu//handle/123456789/4570
dc.identifier.uri doi:10.1016/j.surg.2016.09.031
dc.description.abstract BACKGROUND: Postconditioning may prove to be a suitable method to decrease ischemia-reperfusion injury of intestine after mesenteric arterial occlusion. Toll-like-receptor-4 is involved in the pathophysiology of organ damage after ischemia-reperfusion; therefore, the aim of our study was to investigate the effect of postconditioning on the mucosal expression of toll-like-receptor-4. METHODS: Male Wistar rats (n = 10/group) underwent 60 minutes of superior mesenteric artery occlusion followed by 6 hours of reperfusion in 3 groups: sham-operated, ischemia-reperfusion, and a postconditioned group. Postconditioning was performed by 6 alternating cycles of 10 seconds of reperfusion/reocclusion. Blood and tissue samples were collected at the end of reperfusion. Intestinal histopathologic changes and immunohistochemical expression of mucosal caspase-3, antioxidant status, and protein levels of high-mobility group box-1 and toll-like-receptor-4 were assessed. Immunofluorescent labeling and confocal microscopic analysis of toll-like-receptor-4 were performed. Mucosal and serum levels of interleukin-6 and tumor necrosis factor-alpha protein were measured. RESULTS: Histologic alterations in the postconditioned group were associated with decreased caspase-3 positivity, less toll-like-receptor-4 mRNA, and less protein expression of high-mobility group box-1 and toll-like-receptor-4 in the intestinal villi compared with the ischemia-reperfusion group. Furthermore, a significantly improved antioxidant state of the intestinal mucosa and less mucosal and serum protein levels of interleukin-6 and tumor necrosis factor-alpha were detected in the postconditioned group. CONCLUSION: Small intestinal ischemia-reperfusion injury in male Wistar rats caused by the occlusion of the superior mesenteric artery was ameliorated by the use of postconditioning, showing a more favorable inflammatory response, which may be attributed to the decreased mucosal expression of toll-like-receptor-4.
dc.relation.ispartof urn:issn:0039-6060
dc.title Postconditioning: "Toll-erating" mesenteric ischemia-reperfusion injury?
dc.type Journal Article
dc.date.updated 2017-11-16T14:08:31Z
dc.language.rfc3066 en
dc.identifier.mtmt 3139955
dc.identifier.wos 000398430400015
dc.identifier.pubmed 27842908


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