dc.contributor.author |
Mózes, Miklós |
|
dc.contributor.author |
Szoleczky, Petra |
|
dc.contributor.author |
Rosivall, László |
|
dc.contributor.author |
Kökény, Gábor |
|
dc.date.accessioned |
2018-02-21T10:20:13Z |
|
dc.date.available |
2018-02-21T10:20:13Z |
|
dc.date.issued |
2017 |
|
dc.identifier |
85021692336 |
|
dc.identifier.citation |
pagination=209, 11 pages;
journalVolume=18;
journalIssueNumber=1;
journalTitle=BMC NEPHROLOGY; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/4703 |
|
dc.identifier.uri |
doi:10.1186/s12882-017-0626-2 |
|
dc.description.abstract |
BACKGROUND: Although TGF-ss and the transcription factor Egr-1 play an important role in both kidney fibrosis and in response to acute changes of renal medullary osmolarity, their role under sustained hypo- or hyperosmolar conditions has not been elucidated. We investigated the effects of chronic hypertonicity and hypotonicity on the renal medullary TGF-ss and Egr-1 expression. METHODS: Male adult Sprague Dawley rats (n = 6/group) were treated with 15 mg/day furosemide, or the rats were water restricted to 15 ml/200 g body weight per day. Control rats had free access to water and rodent chow. Kidneys were harvested after 5 days of treament. In cultured inner medullary collecting duct (IMCD) cells, osmolarity was increased from 330 mOsm to 900 mOsm over 6 days. Analyses were performed at 330, 600 and 900 mOsm. RESULTS: Urine osmolarity has not changed due to furosemide treatment but increased 2-fold after water restriction (p < 0.05). Gene expression of TGF-ss and Egr-1 increased by 1.9-fold and 7-fold in the hypertonic medulla, respectively (p < 0.05), accompanied by 6-fold and 2-fold increased c-Fos and TIMP-1 expression, respectively (p < 0.05) and positive immunostaining for TGF-ss and Egr-1 (p < 0.05). Similarly, hyperosmolarity led to overexpression of TGF-ss and Egr-1 mRNA in IMCD cells (2.5-fold and 3.5-fold increase from 330 to 900 mOsm, respectively (p < 0.05)) accompanied by significant c-Fos and c-Jun overexpressions (p < 0.01), and increased Col3a1 and Col4a1 mRNA expression. CONCLUSION: We conclude that both TGF-ss and Egr-1 are upregulated by sustained hyperosmolarity in the rat renal medulla, and it favors the expression of extracellular matrix components. |
|
dc.relation.ispartof |
urn:issn:1471-2369 |
|
dc.title |
Sustained hyperosmolarity increses TGF-beta1 and Egr-1 expression in the rat renal medulla. |
|
dc.type |
Journal Article |
|
dc.date.updated |
2018-02-06T09:14:01Z |
|
dc.language.rfc3066 |
en |
|
dc.identifier.mtmt |
3249099 |
|
dc.identifier.wos |
000404803800001 |
|
dc.identifier.pubmed |
28673338 |
|
dc.contributor.department |
SE/AOK/I/Kórélettani Intézet |
|
dc.contributor.department |
SE/AOK/K/ISZGYK/MTA-SE Gyermekgyógyászati és Nephrológiai Kutatócsoport |
|
dc.contributor.institution |
Semmelweis Egyetem |
|
dc.mtmt.swordnote |
Mózes Miklós and Szoleczky Petra contrubited equally to this work. |
|