dc.contributor.author |
Veres Gábor |
|
dc.contributor.author |
Hegedus P |
|
dc.contributor.author |
Barnucz E |
|
dc.contributor.author |
Zoller R |
|
dc.contributor.author |
Radovits Tamás |
|
dc.contributor.author |
Korkmaz S |
|
dc.contributor.author |
Kolonics F |
|
dc.contributor.author |
Weymann A |
|
dc.contributor.author |
Karck M |
|
dc.contributor.author |
Szabo G |
|
dc.date.accessioned |
2014-11-24T15:40:16Z |
|
dc.date.available |
2014-11-24T15:40:16Z |
|
dc.date.issued |
2013 |
|
dc.identifier |
84880923618 |
|
dc.identifier.citation |
pagination=242-248;
journalVolume=46;
journalIssueNumber=2;
journalTitle=EUROPEAN JOURNAL OF VASCULAR AND ENDOVASCULAR SURGERY; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/474 |
|
dc.identifier.uri |
doi:10.1016/j.ejvs.2013.05.006 |
|
dc.description.abstract |
OBJECTIVE: Based upon the well known protective effect of intracellular cyclic guanosine monophosphate (cGMP) accumulation, we tested the hypothesis that storage solution enriched with optimal concentration of the phosphodiestherase-5 inhibitor vardenafil could provide better protection of vascular grafts against reperfusion injury after long-term cold ischaemic storage. METHODS: Isolated thoracic aorta obtained from rats underwent 24-h cold ischaemic preservation in physiological saline or vardenafil (10(-11) M)-supplemented saline solution. Reperfusion injury was simulated by hypochlorite (200 muM) exposure for 30 minutes. Endothelium-dependent vasorelaxation was assessed, and histopathological and molecular-biological examination of the aortic tissue were performed. RESULTS: Compared with the control group, the saline group showed significantly attenuated endothelium-dependent maximal relaxation (Rmax) to acetylcholine after hypoxia-reoxygenation, which was significantly improved by vardenafil supplementation (Rmax control: 98 +/- 1%; saline: 48 +/- 6%; vardenafil: 75 +/- 4%; p < .05). Vardenafil treatment significantly reduced DNA strand breaks (control: 10.6 +/- 6.2%; saline: 72.5 +/- 4.0%; vardenafil: 14.2 +/- 5.2%; p < .05) and increased cGMP score in the aortic wall (control: 8.2 +/- 0.6; saline: 4.5 +/- 0.3; vardenafil: 6.7 +/- 0.6; p < .05). CONCLUSIONS: Our results support the view that impairment of intracellular cGMP signalling plays a role in the pathogenesis of the endothelial dysfunction induced by cold storage warm reperfusion, which can be effectively reversed by pharmacological phosphodiesterase-5 inhibition. |
|
dc.relation.ispartof |
urn:issn:1078-5884 |
|
dc.title |
Addition of vardenafil into storage solution protects the endothelium in a hypoxia-reoxygenation model |
|
dc.type |
Journal Article |
|
dc.date.updated |
2014-11-10T18:43:28Z |
|
dc.language.rfc3066 |
en |
|
dc.identifier.mtmt |
2372522 |
|
dc.identifier.wos |
000323690900015 |
|
dc.identifier.pubmed |
23751217 |
|
dc.contributor.department |
Szívsebészeti Klinika-névváltozás: 2012- Szívsebészeti Tanszék |
|
dc.contributor.department |
Kardiológiai Központ, Kardiológia Tanszék (névváltozás: 2012-től Kardiológiai Tanszék-Kardiológiai Központ) |
|
dc.mtmt.swordnote |
Megosztott elsőszerzőség: Veres Gábor és Hegedűs Péter között. |
|