Show simple item record Franck Anicet Ditengou Dulceneia Gomes Hugues Nziengui Philip Kochersperger Hanna Lasok Violante Medeiros Ivan A. Paponov Nagy, Szilvia Krisztina Nádai, Tímea Virág Mészáros, Tamás Barnabás, Beáta Beata Izabela Ditengou Katja Rapp Linlin Qi Xugang Li Claude Becker Chuanyou Li Dóczi, Róbert Klaus Palme 2018-10-09T11:34:10Z 2018-10-09T11:34:10Z 2017
dc.identifier.citation pagination=1610-1624; journalVolume=217; journalIssueNumber=4; journalTitle=NEW PHYTOLOGIST;
dc.identifier.uri doi:10.1111/nph.14923
dc.description.abstract Auxin gradients are sustained by series of influx and efflux carriers whose subcellular localization is sensitive to both exogenous and endogenous factors. Recently the localization of the Arabidopsis thaliana auxin efflux carrier PIN-FORMED (PIN) 6 was reported to be tissue-specific and regulated through unknown mechanisms. Here, we used genetic, molecular and pharmacological approaches to characterize the molecular mechanism(s) controlling the subcellular localization of PIN6. PIN6 localizes to endomembrane domains in tissues with low PIN6 expression levels such as roots, but localizes at the plasma membrane (PM) in tissues with increased PIN6 expression such as the inflorescence stem and nectary glands. We provide evidence that this dual localization is controlled by PIN6 phosphorylation and demonstrate that PIN6 is phosphorylated by mitogen-activated protein kinases (MAPKs) MPK4 and MPK6. The analysis of transgenic plants expressing PIN6 at PM or in endomembrane domains reveals that PIN6 subcellular localization is critical for Arabidopsis inflorescence stem elongation post-flowering (bolting). In line with a role for PIN6 in plant bolting, inflorescence stems elongate faster in pin6 mutant plants than in wild-type plants. We propose that PIN6 subcellular localization is under the control of developmental signals acting on tissue-specific determinants controlling PIN6-expression levels and PIN6 phosphorylation.
dc.relation.ispartof urn:issn:0028-646X
dc.title Characterization of auxin transporter PIN6 plasma membrane targeting reveals a function for PIN6 in plant bolting
dc.type Journal Article 2018-02-27T09:45:18Z
dc.language.rfc3066 en
dc.identifier.mtmt 3317796
dc.identifier.pubmed 29218850
dc.contributor.department SE/AOK/I/Orvosi Vegytani, Molekuláris Biológiai és Patobiokémiai Intézet
dc.contributor.institution Semmelweis Egyetem

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