Changes of essential element content and prevention of cisplatin induced oxidative stress in rat liver by CV247 administration

Abstract

Introduction: Metals accumulate mainly in the kidney during cancer therapy with metal complexes. Since liver is the most important organ for chemical metabolism, its function can determine the effectiveness of therapy because of joint side effect of different chemicals in the liver. The aim of the study was to investigate the concentration of the essential elements Ca, Co, Cu, Fe, K, Mg, Mn, Mo, Na, P, S, Zn and antioxidant status in the rat livers following treatment with cisplatin and CV247, a product which contains Cu and Mn gluconate, ascorbic acid and Na salicylate. Methods: Male Wistar rats (n = 40, 175-190g) were randomly divided into 4 groups (n = 10/group). The control group received 1% (w/v)(1) methyl cellulose at 10 mL/kg body weight, p.o.(2) by gastric gavage twice daily for 14 days, while cisplatin was injected i.p.(3) in a single dose of 6.5 mg/kg body weight. The CV247 treated group received 3 mL CV247/kg body weight, p.o. twice daily for 14 days. The fourth group of rats was treated with cisplatin and CV247 in the mentioned doses. Inductively coupled plasma optical emission spectrometry (ICP-OES) was used for measuring Ca, Co, Cu, Fe, K, Mg, Mn, Mo, P, S, Zn in the liver and total scavenger capacity, malondialdehyde and diene conjugate content were also determined. Results: Element depletion was found in the liver of both rat groups treated with CV247, nevertheless the oxidative stress caused by cisplatin was diminished by CV247. Conclusion: These results demonstrate the importance of essential elements, such as Ca and Mg supply during cancer therapy. (C) 2016 Elsevier GmbH. All rights reserved.