dc.contributor.author |
Kádár, Béla |
|
dc.contributor.author |
Kocsis, Béla |
|
dc.contributor.author |
Kristóf, Katalin |
|
dc.contributor.author |
Tóth, Ákos |
|
dc.contributor.author |
Szabó, Dóra |
|
dc.date.accessioned |
2018-06-26T06:31:42Z |
|
dc.date.available |
2018-06-26T06:31:42Z |
|
dc.date.issued |
2015 |
|
dc.identifier |
84953300697 |
|
dc.identifier.citation |
pagination=501-508;
journalVolume=62;
journalIssueNumber=4;
journalTitle=ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/5466 |
|
dc.identifier.uri |
doi:10.1556/030.62.2015.4.12 |
|
dc.description.abstract |
In this study susceptibility to different antimicrobial peptides was investigated on colistin-susceptible and colistin-resistant identical pulsotype strains of KPC-2 producing Klebsiella pneumoniae ST258 as well as colistin-susceptible and colistin-resistant Enterobacter asburiae strains isolated from clinical samples. In our test, bacteria were exposed to 50 mg/ml lactoferrin, lysozyme and protamine - cationic antimicrobial peptides belonging to innate immune system and having structural similarity to polymyxins - in separate reactions. After 18 hours incubation of colonies were counted. 40% of colistin-resistant K. pneumoniae strains and 97% of colistin-susceptible counterpart strains were lysed by protamine whereas 87% and 100% colony forming unit decrease by lysozyme was seen, respectively. In the case of colistin-resistant E. asburiae strains 1 log10 cell count increase were observed after treatment with lysozyme and 1.56 log10 after lactoferrin exposure compared to the initial number whereas the colistin-susceptible showed no relevant cell count increase. Our findings suggest that acquired colistin-resistance in Enterobacteriaceae is associated with tolerance against antimicrobial peptides. |
|
dc.relation.ispartof |
urn:issn:1217-8950 |
|
dc.title |
Effect of antimicrobial peptides on colistin-susceptible and colistin-resistant strains of Klebsiella pneumoniae and Enterobacter asburiae |
|
dc.type |
Journal Article |
|
dc.date.updated |
2018-05-28T06:29:57Z |
|
dc.language.rfc3066 |
en |
|
dc.identifier.mtmt |
2987548 |
|
dc.identifier.wos |
000366946500012 |
|
dc.identifier.pubmed |
26689883 |
|
dc.contributor.department |
SE/AOK/I/Orvosi Mikrobiológiai Intézet |
|
dc.contributor.department |
SE/AOK/I/Laboratóriumi Medicina Intézet |
|
dc.contributor.institution |
Semmelweis Egyetem |
|