dc.contributor.author |
Andocs G |
|
dc.contributor.author |
Meggyesházi, Nóra |
|
dc.contributor.author |
Balogh, Lajos |
|
dc.contributor.author |
Spisák, Sándor |
|
dc.contributor.author |
Maros ME |
|
dc.contributor.author |
Balla, Péter |
|
dc.contributor.author |
Kiszner, Gergő |
|
dc.contributor.author |
Teleki, Ivett |
|
dc.contributor.author |
Kővágó, Csaba |
|
dc.contributor.author |
Krenács, Tibor |
|
dc.date.accessioned |
2018-06-11T08:53:32Z |
|
dc.date.available |
2018-06-11T08:53:32Z |
|
dc.date.issued |
2015 |
|
dc.identifier |
84914672760 |
|
dc.identifier.citation |
pagination=37-46;
journalVolume=20;
journalTitle=CELL STRESS & CHAPERONES; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/5546 |
|
dc.identifier.uri |
doi:10.1007/s12192-014-0523-6 |
|
dc.description.abstract |
In modulated electrohyperthermia (mEHT) the enrichment of electric field and the concomitant heat can selectively induce cell death in malignant tumors as a result of elevated glycolysis, lactate production (Warburg effect), and reduced electric impedance in cancer compared to normal tissues. Earlier, we showed in HT29 colorectal cancer xenografts that the mEHT-provoked programmed cell death was dominantly caspase independent and driven by apoptosis inducing factor activation. Using this model here, we studied the mEHT-related cell stress 0-, 1-, 4-, 8-, 14-, 24-, 48-, 72-, 120-, 168- and 216-h post-treatment by focusing on damage-associated molecular pattern (DAMP) signals. Significant cell death response upon mEHT treatment was accompanied by the early upregulation (4-h post-treatment) of heat shock protein (Hsp70 and Hsp90) mRNA levels. In situ, the treatment resulted in spatiotemporal occurrence of a DAMP protein signal sequence featured by the significant cytoplasmic to cell membrane translocation of calreticulin at 4 h, Hsp70 between 14 and 24 h and Hsp90 between 24- and 216-h post-treatment. The release of high-mobility group box1 protein (HMGB1) from tumor cell nuclei from 24-h post-treatment and its clearance from tumor cells by 48 h was also detected. Our results suggest that mEHT treatment can induce a DAMP-related signal sequence in colorectal cancer xenografts that may be relevant for promoting immunological cell death response, which need to be further tested in immune-competent animals. |
|
dc.relation.ispartof |
urn:issn:1355-8145 |
|
dc.title |
Upregulation of heat shock proteins and the promotion of damage-associated molecular pattern signals in a colorectal cancer model by modulated electrohyperthermia |
|
dc.type |
Journal Article |
|
dc.date.updated |
2018-06-06T12:32:47Z |
|
dc.language.rfc3066 |
en |
|
dc.identifier.mtmt |
2708818 |
|
dc.identifier.wos |
000345994600005 |
|
dc.identifier.pubmed |
24973890 |
|
dc.contributor.department |
SE/AOK/I/I. Sz. Patológiai és Kísérleti Rákkutató Intézet |
|
dc.contributor.department |
SE/AOK/I/IISZPI/MTA-SE Molekuláris Onkológia Kutatócsoport |
|
dc.contributor.department |
Gyógyszertani és méregtani tanszék [2016.06.30] |
|
dc.contributor.institution |
Semmelweis Egyetem |
|
dc.contributor.institution |
Szent István Egyetem |
|