dc.contributor.author |
Veres, Gábor |
|
dc.contributor.author |
Hegedűs, Péter |
|
dc.contributor.author |
Barnucz, Enikö |
|
dc.contributor.author |
Zoller R |
|
dc.contributor.author |
Klein S |
|
dc.contributor.author |
Schmidt H |
|
dc.contributor.author |
Radovits, Tamás |
|
dc.contributor.author |
Korkmaz S |
|
dc.contributor.author |
Karck M |
|
dc.contributor.author |
Szabó, Gábor Balázs |
|
dc.date.accessioned |
2018-09-11T12:50:35Z |
|
dc.date.available |
2018-09-11T12:50:35Z |
|
dc.date.issued |
2015 |
|
dc.identifier |
84928888331 |
|
dc.identifier.citation |
pagination=e0124025, 13;
journalVolume=10;
journalIssueNumber=4;
journalTitle=PLOS ONE; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/5814 |
|
dc.identifier.uri |
doi:10.1371/journal.pone.0124025 |
|
dc.description.abstract |
BACKGROUND: Although, ischemia/reperfusion induced vascular dysfunction has been widely described, no comparative study of in vivo- and in vitro-models exist. In this study, we provide a direct comparison between models (A) ischemic storage and in-vitro reoxygenation (B) ischemic storage and in vitro reperfusion (C) ischemic storage and in-vivo reperfusion. METHODS AND RESULTS: Aortic arches from rats were stored for 2 hours in saline. Arches were then (A) in vitro reoxygenated (B) in vitro incubated in hypochlorite for 30 minutes (C) in vivo reperfused after heterotransplantation (2, 24 hours and 7 days reperfusion). Endothelium-dependent and independent vasorelaxations were assessed in organ bath. DNA strand breaks were assessed by TUNEL-method, mRNA expressions (caspase-3, bax, bcl-2, eNOS) by quantitative real-time PCR, proteins by Western blot analysis and the expression of CD-31 by immunochemistry. Endothelium-dependent maximal relaxation was drastically reduced in the in-vivo models compared to ischemic storage and in-vitro reperfusion group, and no difference showed between ischemic storage and control group. CD31-staining showed significantly lower endothelium surface ratio in-vivo, which correlated with TUNEL-positive ratio. Increased mRNA and protein levels of pro- and anti-apoptotic gens indicated a significantly higher damage in the in-vivo models. CONCLUSION: Even short-period of ischemia induces severe endothelial damage (in-vivo reperfusion model). In-vitro models of ischemia-reperfusion injury can be limitedly suited for reliable investigations. Time course of endothelial stunning is also described. |
|
dc.relation.ispartof |
urn:issn:1932-6203 |
|
dc.title |
Endothelial dysfunction of bypass graft: Direct comparison of In Vitro and In Vivo models of ischemia-reperfusion injury |
|
dc.type |
Journal Article |
|
dc.date.updated |
2018-07-13T11:27:59Z |
|
dc.language.rfc3066 |
en |
|
dc.identifier.mtmt |
2885971 |
|
dc.identifier.wos |
000353015800128 |
|
dc.identifier.pubmed |
25875813 |
|
dc.contributor.department |
SE/AOK/K/VAROSMAJOR_SZÍVÉRGYÓGY/Kardiológia Központ - Kardiológiai Tanszék [2017.10.31] |
|
dc.contributor.institution |
Semmelweis Egyetem |
|