Show simple item record Székács, Inna Mike-Kaszás, Nóra Gróf, Pál Erdelyi K Szendro I Mihalik B Pataki A Antoni FA Madarász, Emilia 2018-09-04T11:32:18Z 2018-09-04T11:32:18Z 2013
dc.identifier 84892421997
dc.identifier.citation pagination=e81398, pages 11; journalVolume=8; journalIssueNumber=12; journalTitle=PLOS ONE;
dc.identifier.uri doi:10.1371/journal.pone.0081398
dc.description.abstract Optical waveguide lightmode spectroscopic (OWLS) techniques were probed for monitoring ion permeation through channels incorporated into artificial lipid environment. A novel sensor set-up was developed by depositing liposomes or cell-derived membrane fragments onto hydrophilic polytetrafluoroethylene (PTFE) membrane. The fibrous material of PTFE membrane could entrap lipoid vesicles and the water-filled pores provided environment for the hydrophilic domains of lipid-embedded proteins. The sensor surface was kept clean from the lipid holder PTFE membrane by a water- and ion-permeable polyethylene terephthalate (PET) mesh. The sensor set-up was tested with egg yolk lecithin liposomes containing gramicidin ion channels and with cell-derived membrane fragments enriched in GABA-gated anion channels. The method allowed monitoring the move of Na(+) and organic cations through gramicidin channels and detecting the Cl(-)-channel functions of the (alpha5beta2gamma2) GABAA receptor in the presence or absence of GABA and the competitive GABA-blocker bicuculline.
dc.relation.ispartof urn:issn:1932-6203
dc.title Optical waveguide lightmode spectroscopic techniques for investigating membrane-bound ion channel activities.
dc.type Journal Article 2018-07-20T07:46:50Z
dc.language.rfc3066 en
dc.identifier.mtmt 2506863
dc.identifier.wos 000328707400016
dc.identifier.pubmed 24339925
dc.contributor.department MTA Kísérleti Orvostudományi Kutatóintézet
dc.contributor.department SE/AOK/I/Biofizikai és Sugárbiológiai Intézet
dc.contributor.institution MTA Kísérleti Orvostudományi Kutatóintézet
dc.contributor.institution Semmelweis Egyetem

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