dc.contributor.author |
Madonna R |
|
dc.contributor.author |
Geng YJ |
|
dc.contributor.author |
Shelat H |
|
dc.contributor.author |
Ferdinandy, Péter |
|
dc.contributor.author |
De Caterina R |
|
dc.date.accessioned |
2018-08-29T14:27:56Z |
|
dc.date.available |
2018-08-29T14:27:56Z |
|
dc.date.issued |
2014 |
|
dc.identifier |
84908430616 |
|
dc.identifier.citation |
pagination=2266-2275;
journalVolume=1842;
journalIssueNumber=11;
journalTitle=BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/6171 |
|
dc.identifier.uri |
doi:10.1016/j.bbadis.2014.07.030 |
|
dc.description.abstract |
Background and objective: Hyperglycemia leads to adaptive cell responses in part due to hyperosmolarity. In endothelial and epithelial cells, hyperosmolarity induces aquaporin-1 (AQP1) which plays a role in cytoskeletal remodeling, cell proliferation and migration. Whether such impairments also occur in human induced pluripotent stem cells (iPS) is not known. We therefore investigated whether high glucose-induced hyperosmolarity impacts proliferation, migration, expression of pluripotency markers and actin skeleton remodeling in iPS cells in an AQP1-dependent manner. Methods and results: Human iPS cells were generated from skin fibroblasts by lentiviral transduction of four reprogramming factors (Oct4, Sox2, Klf4, c-Myc). After reprogramming, iPS cells were characterized by their adaptive responses to high glucose-induced hyperosmolarity by incubation with 5.5mmol/L glucose, high glucose (HG) at 30.5mM, or with the hyperosmolar control mannitol (HM). Exposure to either HG or HM increased the expression of AQP1. AQP1 co-immunoprecipitated with beta-catenin. HG and HM induced the expression of beta-catenin. Under these conditions, iPS cells showed increased ratios of F-actin to G-actin and formed increased tubing networks. Inhibition of AQP1 with small interfering RNA (siRNA) reverted the inducing effects of HG and HM. Conclusions: High glucose enhances human iPS cell proliferation and cytoskeletal remodeling due to hyperosmolarity-induced upregulation of AQP1. |
|
dc.relation.ispartof |
urn:issn:0925-4439 |
|
dc.title |
High glucose-induced hyperosmolarity impacts proliferation, cytoskeleton remodeling and migration of human induced pluripotent stem cells via aquaporin-1 |
|
dc.type |
Journal Article |
|
dc.date.updated |
2018-08-27T17:06:23Z |
|
dc.language.rfc3066 |
en |
|
dc.identifier.mtmt |
2718848 |
|
dc.identifier.wos |
000343844800022 |
|
dc.identifier.pubmed |
25108283 |
|
dc.contributor.department |
SE/AOK/I/Farmakológiai és Farmakoterápiás Intézet |
|
dc.contributor.institution |
Semmelweis Egyetem |
|