dc.contributor.author |
Hrubi E |
|
dc.contributor.author |
Imre, László |
|
dc.contributor.author |
Robaszkiewicz A |
|
dc.contributor.author |
Virág, László |
|
dc.contributor.author |
Kerenyi F |
|
dc.contributor.author |
Nagy, Krisztina |
|
dc.contributor.author |
Varga, Gábor |
|
dc.contributor.author |
Jenei, Attila |
|
dc.contributor.author |
Hegedűs, Csaba |
|
dc.date.accessioned |
2018-10-02T09:59:41Z |
|
dc.date.available |
2018-10-02T09:59:41Z |
|
dc.date.issued |
2018 |
|
dc.identifier |
85041892889 |
|
dc.identifier.citation |
pagination=139-148;
journalVolume=31;
journalIssueNumber=2;
journalTitle=HUMAN CELL: THE OFFICIAL JOURNAL OF THE JAPAN HUMAN CELL SOCIETY; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/6432 |
|
dc.identifier.uri |
doi:10.1007/s13577-018-0202-5 |
|
dc.description.abstract |
Bone morphogenetic protein-2 (BMP-2), is a potential factor to enhance osseointegration of dental implants. However, the appropriate cellular system to investigate the osteogenic effect of BMP-2 in vitro in a standardized manner still needs to be defined. The aim of this study was to examine the effect of BMP-2 on the cell proliferation and osteogenic differentiation of human osteogenic progenitors of various origins: dental pulp stem cells (DPSC), human osteosarcoma cell line (Saos-2) and human embryonic palatal mesenchymal cell line (HEPM). For induction of osteogenic differentiation, cell culture medium was supplemented with BMP-2 homodimer alone or in combination with conventionally used differentiation inducing agents. Differentiation was monitored for 6-18 days. To assess differentiation, proliferation rate, alkaline phosphatase activity, calcium deposition and the expression level of osteogenic differentiation marker genes (Runx2, BMP-2) were measured. BMP-2 inhibited cell proliferation in a concentration and time-dependent manner. In a concentration which caused maximal cell proliferation, BMP-2 did not induce osteogenic differentiation in any of the tested systems. However, it had a synergistic effect with the osteoinductive medium in both DPSC and Saos-2, but not in HEPM cells. We also found that the differentiation process was faster in Saos-2 than in DPSCs. Osteogenic differentiation could not be induced in the osteoblast progenitor HEPM cells. Our data suggest that in a concentration that inhibits proliferation the differentiation inducing effect of BMP-2 is evident only in the presence of permissive osteoinductive components. beta-glycerophosphate, was identified interacting with BMP-2 in a synergistic manner. |
|
dc.relation.ispartof |
urn:issn:0914-7470 |
|
dc.title |
Diverse effect of BMP-2 homodimer on mesenchymal progenitors of different origin |
|
dc.type |
Journal Article |
|
dc.date.updated |
2018-09-13T09:15:36Z |
|
dc.language.rfc3066 |
en |
|
dc.identifier.mtmt |
3352923 |
|
dc.identifier.wos |
000427606500006 |
|
dc.identifier.pubmed |
29442285 |
|
dc.contributor.department |
DE/ÁOK/Orvosi Vegytani Intézet |
|
dc.contributor.department |
DE/ÁOK/Biofizikai és Sejtbiológiai Intézet |
|
dc.contributor.department |
SE/FOK/Orálbiológiai Tanszék |
|
dc.contributor.institution |
Debreceni Egyetem |
|
dc.contributor.institution |
Semmelweis Egyetem |
|