Show simple item record Osváth Szabolcs Herényi Levente Agócs Gergely Kis-Petik Katalin Kellermayer Miklós 2018-10-13T10:45:37Z 2018-10-13T10:45:37Z 2018
dc.identifier.citation pagination=874-880; journalVolume=115; journalIssue=5; journalTitle=BIOPHYSICAL JOURNAL;
dc.identifier.uri doi:10.1016/j.bpj.2018.07.034
dc.description.abstract The living cell is characterized by a myriad of parallel intracellular transport processes. Simultaneously capturing their global features across multiple temporal and spatial scales is a nearly unsurmountable task. Here we present a method that enables the microscopic imaging of the entire spectrum of intracellular transport on a broad time scale without the need for prior labeling. We show that from the time-dependent fluctuation of pixel intensity, in either bright-field or phase-contrast microscopic images, a scaling factor can be derived that reflects the local Hurst coefficient (H), the value of which reveals the microscopic mechanisms of intracellular motion. The Hurst coefficient image of the interphase cell displays an unexpected, overwhelming superdiffusion (H > 0.5) in the cytoplasm and subdiffusion (H < 0.5) in the nucleus, and provides unprecedented sensitivity in detecting transport processes associated with the living state.
dc.relation.ispartof urn:issn:0006-3495
dc.title Label-free Multiscale Transport Imaging of the Living Cell
dc.type Journal Article 2018-09-14T09:01:39Z
dc.language.rfc3066 en
dc.identifier.mtmt 3405416
dc.identifier.pubmed 30126614
dc.contributor.department SE/AOK/I/Biofizikai és Sugárbiológiai Intézet
dc.contributor.institution Semmelweis Egyetem
dc.mtmt.swordnote FELTÖLTŐ: Haluszka Dóra -

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