dc.contributor.author |
Pertici I |
|
dc.contributor.author |
Bongini L |
|
dc.contributor.author |
Melli L |
|
dc.contributor.author |
Bianchi G |
|
dc.contributor.author |
Salvi L |
|
dc.contributor.author |
Falorsi G |
|
dc.contributor.author |
Squarci C |
|
dc.contributor.author |
Bozó, Tamás |
|
dc.contributor.author |
Cojoc D |
|
dc.contributor.author |
Kellermayer, Miklós |
|
dc.contributor.author |
Lombardi V |
|
dc.contributor.author |
Bianco P |
|
dc.date.accessioned |
2018-10-08T06:38:21Z |
|
dc.date.available |
2018-10-08T06:38:21Z |
|
dc.date.issued |
2018 |
|
dc.identifier.citation |
pagination=3532, pages: 10;
journalVolume=9;
journalIssueNumber=1;
journalTitle=NATURE COMMUNICATIONS; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/6450 |
|
dc.identifier.uri |
doi:10.1038/s41467-018-06073-9 |
|
dc.description.abstract |
The contraction of striated muscle (skeletal and cardiac muscle) is generated by ATP-dependent interactions between the molecular motor myosin II and the actin filament. The myosin motors are mechanically coupled along the thick filament in a geometry not achievable by single-molecule experiments. Here we show that a synthetic one-dimensional nanomachine, comprising fewer than ten myosin II dimers purified from rabbit psoas, performs isometric and isotonic contractions at 2 mM ATP, delivering a maximum power of 5 aW. The results are explained with a kinetic model fitted to the performance of mammalian skeletal muscle, showing that the condition for the motor coordination that maximises the efficiency in striated muscle is a minimum of 32 myosin heads sharing a common mechanical ground. The nanomachine offers a powerful tool for investigating muscle contractile-protein physiology, pathology and pharmacology without the potentially disturbing effects of the cytoskeletal-and regulatory-protein environment. |
|
dc.relation.ispartof |
urn:issn:2041-1723 |
|
dc.title |
A myosin II nanomachine mimicking the striated muscle |
|
dc.type |
Journal Article |
|
dc.date.updated |
2018-09-14T09:05:51Z |
|
dc.language.rfc3066 |
en |
|
dc.identifier.mtmt |
3411348 |
|
dc.identifier.pubmed |
30166542 |
|
dc.contributor.department |
SE/AOK/I/Biofizikai és Sugárbiológiai Intézet |
|
dc.contributor.institution |
Semmelweis Egyetem |
|
dc.mtmt.swordnote |
FELTÖLTŐ: Haluszka Dóra - haluszka.dora@med.semmelweis-univ.hu |
|