Show simple item record Veres Dániel Máthé Domokos Futó Ildikó Horváth Ildikó Balázs Ákos Karlinger Kinga Szigeti Krisztián 2018-10-01T16:08:18Z 2018-10-01T16:08:18Z 2014
dc.identifier 84896395371
dc.identifier.citation pagination=167-172; journalVolume=16; journalIssueNumber=2; journalTitle=MOLECULAR IMAGING AND BIOLOGY;
dc.identifier.uri doi:10.1007/s11307-013-0679-y
dc.description.abstract PURPOSE: The aim of this paper is to present a simple and quantitative data analysis method with a new potential in the application of liver single-photon emission computed tomography (SPECT) imaging. We have established quantitative SPECT/computed tomography (CT) in vivo imaging protocols for determination of liver tumor burden based on the known role of Kupffer cells in cancer of the liver. PROCEDURES: As it is also known that functional Kupffer cells accumulate particulate material contained in the arterial blood of liver supply, we used radiolabeled macro-aggregated albumin particles ([99mTc]-MAA) injected intravenously to image liver disease. Quantification of cold spot liver lesion imaging was also a general objective. METHODS: We examined a healthy control group (BALB/C mice, n = 6) and group of induced hepatocellular carcinoma (HCC, matrilin-2 transgenic KO mice, n = 9), where hepatocellular carcinoma was induced by diethylnitrosamine. We used [99mTc]-MAA as radiopharmaceutical for liver SPECT imaging in a small animal SPECT/CT system. A liver radioactivity overview map was generated. Segmentation of the liver was calculated by Otsu thresholding method. Based on the segmentation the radioactivity volume and the summarized liver activity were determined. RESULTS: Tumor burden of the livers was quantitatively determined by creating parametric data from the resulting volumetric maps. Ex vivo liver mass data were applied for the validation of in vivo measurements. An uptake with cold spots as tumors was observed in all diseased animals in SPECT/CT scans. Isotope-labeled particle uptake (standardized uptake concentration) of control (median 0.33) and HCC (median 0.18) groups was significantly different (p = 0.0015, Mann Whitney U test). CONCLUSION: A new potential application of [99mTc]-MAA was developed and presents a simple and very effective means to quantitatively characterize liver cold spot lesions resulting from Kupffer cell dysfunctions as a consequence of tumor burden.
dc.relation.ispartof urn:issn:1536-1632
dc.title Quantitative Liver Lesion Volume Determination by Nanoparticle-Based SPECT
dc.type Journal Article 2018-09-14T10:12:20Z
dc.language.rfc3066 en
dc.identifier.mtmt 2420403
dc.identifier.wos 000332828200004
dc.identifier.pubmed 23996677
dc.contributor.department SE/AOK/K/I. Sz. Sebészeti Klinika
dc.contributor.department SE/AOK/I/Biofizikai és Sugárbiológiai Intézet
dc.contributor.department SE/AOK/K/Radiológiai és Onkoterápiás Klinika
dc.contributor.institution Semmelweis Egyetem
dc.mtmt.swordnote FELTÖLTŐ: Haluszka Dóra -

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