dc.contributor.author |
Stoddard Michelina |
|
dc.contributor.author |
Huang Cong |
|
dc.contributor.author |
Enyedi, Balázs |
|
dc.contributor.author |
Niethammer Philipp |
|
dc.date.accessioned |
2019-03-20T15:41:25Z |
|
dc.date.available |
2019-03-20T15:41:25Z |
|
dc.date.issued |
2019 |
|
dc.identifier.citation |
journalVolume=9;journalIssueNumber=1;journalTitle=SCIENTIFIC REPORTS;pagerange=28, pages: 11;journalAbbreviatedTitle=SCI REP; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/6811 |
|
dc.identifier.uri |
doi:10.1038/s41598-018-36771-9 |
|
dc.description.abstract |
Studying early immune responses to organ damage in situ requires animal models amenable to intravital imaging. Here, we used transparent zebrafish larvae, a powerful animal model for innate immunity, to measure leukocyte recruitment to damaged livers. Bath application of metronidazole (Mtz) to fish expressing nitroreductase (NTR) under a liver-specific promoter damaged the organ within 24 hours causing oxidative stress, distorted liver morphology, accumulation of TUNEL-positive cells, and transcriptional upregulation of apoptotic and antioxidant genes. Inflammatory gene transcription in damaged hepatocytes was attenuated. In line with predominant apoptosis, macrophages were massively recruited into Mtz/NTR-damaged livers. By contrast, neutrophil infiltration was more variable and delayed, consistent with less abundant necrosis and an attenuated inflammatory capacity of damaged hepatocytes. |
|
dc.relation.ispartof |
urn:issn:2045-2322 |
|
dc.title |
Live imaging of leukocyte recruitment in a zebrafish model of chemical liver injury |
|
dc.type |
Journal Article |
|
dc.date.updated |
2019-02-28T11:41:17Z |
|
dc.language.rfc3066 |
en |
|
dc.rights.holder |
NULL |
|
dc.identifier.mtmt |
30391410 |
|
dc.identifier.wos |
000455352400011 |
|
dc.identifier.pubmed |
30631093 |
|
dc.contributor.department |
SE/AOK/I/Élettani Intézet |
|
dc.contributor.institution |
Semmelweis Egyetem |
|