Show simple item record Stoddard Michelina Huang Cong Enyedi, Balázs Niethammer Philipp 2019-03-20T15:41:25Z 2019-03-20T15:41:25Z 2019
dc.identifier.citation journalVolume=9;journalIssueNumber=1;journalTitle=SCIENTIFIC REPORTS;pagerange=28, pages: 11;journalAbbreviatedTitle=SCI REP;
dc.identifier.uri doi:10.1038/s41598-018-36771-9
dc.description.abstract Studying early immune responses to organ damage in situ requires animal models amenable to intravital imaging. Here, we used transparent zebrafish larvae, a powerful animal model for innate immunity, to measure leukocyte recruitment to damaged livers. Bath application of metronidazole (Mtz) to fish expressing nitroreductase (NTR) under a liver-specific promoter damaged the organ within 24 hours causing oxidative stress, distorted liver morphology, accumulation of TUNEL-positive cells, and transcriptional upregulation of apoptotic and antioxidant genes. Inflammatory gene transcription in damaged hepatocytes was attenuated. In line with predominant apoptosis, macrophages were massively recruited into Mtz/NTR-damaged livers. By contrast, neutrophil infiltration was more variable and delayed, consistent with less abundant necrosis and an attenuated inflammatory capacity of damaged hepatocytes.
dc.relation.ispartof urn:issn:2045-2322
dc.title Live imaging of leukocyte recruitment in a zebrafish model of chemical liver injury
dc.type Journal Article 2019-02-28T11:41:17Z
dc.language.rfc3066 en
dc.rights.holder NULL
dc.identifier.mtmt 30391410
dc.identifier.wos 000455352400011
dc.identifier.pubmed 30631093
dc.contributor.department SE/AOK/I/Élettani Intézet
dc.contributor.institution Semmelweis Egyetem

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